半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2N18 is a Rabbit recombinant monoclonal antibody that specifically detects Grainyhead-like protein 2 homolog (GRHL2). It targets an epitope within 15 amino acids from the internal region.
免疫原
KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Grainyhead-like protein 2 homolog (GRHL2).
应用
Quality Control Testing
Evaluated by Western Blotting in Mouse testis tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GRHL2 in Mouse testis tissue lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GRHL2 in lysates from human testis tissue and KNRK cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GRHL2 in human skin tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GRHL2 in lysates from A431, HeLa, HepG2, and NIH 3T3 cells.
Affinity Binding Assay: A representative lot of this antibody bound GRHL2 with a KD of 2.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Grainyhead-like protein 2 homolog (UniProt: Q6ISB3; also known as Brother of mammalian grainyhead, Transcription factor CP2-like 3, GRHL2) is encoded by the GRHL2 (also known as BOM, TFCP2L3) gene (Gene ID: 79977) in human. GRHL2 is a homodimeric transcription factor that plays an important role in primary neurulation and in epithelial development. It can also exist in heterodimeric form with GRHL1 or GHRL3. It is also involved in wound healing, maintenance of epidermal integrity, and epithelial-to-mesenchymal transition. It binds directly to the consensus DNA sequence 5′-AACCGGTT-3′ acting as an activator and repressor on distinct target genes. It is present at cell-cell contact sites and its expression is observed to be high in keratinocytes, placenta, prostrate, brain, and kidney. It is expressed in proliferating cells and its expression is reduced during senescence. During embryogenesis, it plays unique and cooperative roles with GRHL3 in establishing distinct zones of primary neurulation. It is shown to be essential for closure 3 (rostral end of the forebrain), closure 2 (forebrain/midbrain boundary), and posterior neuropore closure. In keratinocytes, it plays a role in telomerase activation during cellular proliferation and regulates TERT expression by binding to TERT promoter region and inhibiting DNA methylation at the 5′-CpG island, possibly by interfering with DNMT1 enzyme activity. Overexpression of GRHL2 promotes cell growth and colony formation, and simultaneously suppresses cell migration in non-small cell lung carcinoma cells. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pan, X., et al. (2017). Am. J. Transl. Res. 9(9); 4217- 4226; Chen, W., et al. (2010). J. Biol. Chem. 285 (52); 40852-40863).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | rabbit |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 2N18, recombinant monoclonal |
| 描述 | recombinant, expressed in HEK 293 cells |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 71.11 kDa observed mol wt ~75 kDa |
| 纯化方式 | using Protein A |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | canine, feline, bovine, monkey |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry. |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 表位序列 | Internal |
| Protein ID登记号 | NP_079191 |
| UniProt登记号 | Q6ISB3 |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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