半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 16D21 is a a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human and murine GLUT-1. It targets an epitope with in 12 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Solute carrier family 2, facilitated glucose transporter member 1 (GLUT-1).
应用
Anti-GLUT-1, CT, clone 16D21 ZooMAb, Cat. No. ZRB071401, is arecombinant Rabbit Monoclonal Antibody that specifically targets GLUT1 and has been tested for use in Immunocytochemistry, Immunofluorescence., Immunohistochemistry, and Western Blotting,
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected GLUT-1 in human placenta tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GLUT-1 in human placenta frozen sections.
Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected GLUT-1 in NIH3T3 and HepG2 cells.
目标描述
Solute carrier family 2, facilitated glucose transporter member 1 (UniProt: P11166; also known as Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter) is encoded by the SLC2A1 (also known as GLUT1) gene (Gene ID: 6513) in human. Glucose transporters are a family of integral membrane proteins that facilitative glucose uptake by cells. Seven different glucose transport proteins have been described that are designated as GLUT-1 to 7. GLUT-1 is a highly conserved; ubiquitously distributed, multi-pass membrane protein that is responsible for constitutive glucose uptake. It displays very broad substrate specificity and can transport a wide range of aldoses including both pentoses and hexoses. It is the predominant glucose transporter in embryonic and fetal tissues. In many organs, GLUT-1 is concentrated in endothelial cells of blood-tissue barriers. Hence, it has a specialized role to shuttle glucose between blood and organs that have limited access to small solutes via passive diffusion. It is also abundant in the mammalian erythrocyte membrane where it can rapidly equilibrate glucose between the cytoplasm of the erythrocyte and the blood plasma. GLUT-1 levels are reported to be frequently upregulated during tumorigenesis. Mutations in SLC2A1 gene are linked to GLUT-1 deficiency syndrome 1 and 2 that are characterized by infantile-onset epileptic encephalopathy, delayed development, microcephaly, and motor incoordination, and paroxysmal exercise-induced dyskinesia. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
生物来源 | rabbit (recombinant) |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 16D21, monoclonal recombinant monoclonal |
半岛bd体育手机客户端 线 | ZooMAb?learn more |
形式 | lyophilized |
分子量 | calculated mol wt 54.08 kDa observed mol wt ~52 kDa |
species reactivity | human, mouse |
species reactivity (predicted by homology) | bovine, rat |
包装 | antibody small pack of 25 μL |
环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry. |
增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
technique(s) | immunocytochemistry: 1:100-1:250 immunofluorescence: suitable using 1:100 immunohistochemistry: 1:100-1:250 western blot: suitable using 1:1,000 |
同位素/亚型 | IgG |
UniProt登记号 | P11166 |
环保替代半岛bd体育手机客户端 分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
Gene Information | human ... SLC2A1;GLUT1(6513) |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich