半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 1K20 is a ZooMAb?rabbit recombinant monoclonal antibody that detects Glutamate receptors 2 and 3. It targets an epitope within 13 amino acids from the C-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal, cytoplasmic domain of human Glutamate receptor 2.
应用
Anti-GluR-2, clone 1K20 ZooMAb?, Cat. No. ZRB1923, is a recombinant Rabbit monoclonal antibody that targets Glutamate receptors 2 and 3 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GluR-2 in Rat brain tissue lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GluR-2 in human and mouse brain tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GluR-2 in E18 rat cortical cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GluR-2 in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Glutamate receptor 2 (UniProt: P42262; also known GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, Glutamate receptor ionotropic AMPA 2, GluA2) is encoded by the GRIA2 (also known as GLUR2) gene (Gene ID: 2891) in human. Glutamate receptors are synaptic receptors located primarily on the membranes of neuronal cells. These receptors play important roles in neural communication, memory formation, learning, and regulation by mediating glutamate-dependent postsynaptic excitation. Glutamate receptors are divided into two types, ionotropic glutamate receptors (iGluRs) and metabotropic glutamate receptors (mGluRs). iGluRs form ion channel pores upon glutamate binding, while mGluRs indirectly activate ion channels on the plasma membrane via G protein-coupled signaling cascade. Although all iGluRs and mGluRs bind glutamate, they do exhibit different binding activities toward chemical ligands, such as NMDA, kainate, AMPA, L-AP4, ACPD, L-QA, and such distinction is used to divide these receptors further into subtypes. GluR-2 is a multi-pass membrane AMPA type iGluR with two extracellular domains, four transmembrane domains, and three cytoplasmic domains. It is initially synthesized with a signal peptide sequence (aa. 1-24) that is subsequently cleaved off to generate the mature receptor. GluR2 can exist as homotetramer or a heterotetramer that is formed by dimerization of dimers. It is palmitoylated in its resting state and is depalmitoylated upon glutamate stimulation. Palmitoylation at cysteine 610 leads to its Golgi retention and decreased cell surface expression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | rabbit |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1K20, recombinant monoclonal |
| 描述 | recombinant, expressed in HEK 293 cells |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 98.82 and 101.16 kDa observed mol wt ~105 kDa |
| species reactivity | human, rat, mouse |
| species reactivity (predicted by homology) | Xenopus, bovine |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 表位序列 | C-terminal |
| Protein ID登记号 | NP_000817.2 |
| UniProt登记号 | P42262 |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
Sigma-Aldrich
m.cnreagent.com
