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Anti-GFAP Antibody, clone 2E8, ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB2383
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-GFAP Antibody, clone 2E8, ZooMAb ?Rabbit Monoclonal
GFAP
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2E8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glial fibrilary acidic protein (GFAP). It targets an epitope with in 11 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal region of human Glial fibrilary acidic protein (GFAP).

应用

Anti-GFAP, clone 2E8, ZooMAb, Cat. No. ZRB2383, is a recombinant Rabbit Monoclonal Antibody that specifically targets Glial fibrillary acidic protein (GFAP) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunfluorescence, and Western Blotting.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GFAP in human brain and rat brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GFAP in C6 glioblastoma cells and primary cultures of rat glial precursor cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GFAP in rat brain and human brain tissue sections.

目标描述

Glial fibrillary acidic protein (UniProt: P14136; also known as GFAP) is encoded by the GFAP gene (Gene ID: 2670) in human. GFAP is a class-III intermediate filament that serves as a cell-specific marker that can distinguish astrocytes from other glial cells. GFAP is not detected outside the central nervous system. It is expressed in cells that lack fibronectin. GFAP is a good marker of astroglial injury where its levels are consistently upregulated. GFAP levels are also shown to be upregulated with age in rodents and humans. Hence, it can serve as a surrogate marker of aging in the brain. GFAP has a relatively slow metabolic turnover rate, which is consistent with its structural role. GFAP is composed of three distinct regions: an N-terminal head region, a central ROD region, and a C-terminal tail region. The ROD regions are highly conserved among intermediate filament proteins. However, the size and amino acid sequences of the head and tail regions can vary widely. Three different isoforms of GFAP have been described that are produced by alternative splicing. Mutations in GFAP gene are known to cause Alexander disease that is characterized by progressive failure of central myelination, usually leading to death within the first decade. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 rabbit (recombinant)
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 2E8, monoclonal
recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 49.88 kDa
observed mol wt ~51 kDa
species reactivity rat, human, mouse
包装 antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about thePrinciples of Green Chemistry.
增强验证 recombinant expression
Learn more aboutAntibody Enhanced Validation
technique(s) immunocytochemistry: suitable using 1:100
immunofluorescence: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
western blot: suitable using 1:1,000
同位素/亚型 IgG
UniProt登记号 P14136
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃
Gene Information human ... GFAP(2670)

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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