半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
特异性
Clone TE-7 is a ZooMAb?Mouse recombinant monoclonal antibody that detects fibroblasts in various human tissues.
免疫原
Human thymic stroma cells.
应用
Quality Control Testing
Evaluated by Immunocytochemistry in human foreskin fibroblasts.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Fibroblast in human foreskin fibroblasts.
Tested applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected human foreskin fibroblasts.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibroblast in human skeletal muscle and human skin tissue sections.
Immunocytochemistry Analysis: A representative lot detected Fibroblast in Immunocytochemistry applications (Goodpaster, T., et. al. (2008). J Histochem Cytochem. 56(4):347-58).
Immunohistochemistry Applications: A representative lot detected Fibroblast in Immunohistohemistry applications (Goodpaster, T., et. al. (2008). J. Histochem. Cytochem. 56(4):347-58).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Fibroblasts, spindle-shaped cells, are derived from undifferentiated mesenchymal cells and are the most common cells of connective tissue in animals. Their main function is to maintain the structural integrity of connective tissues by continuously secreting precursors of ECM. They are capable of both synthesis and degradation of extracellular matrix (ECM). Unstimulated fibroblasts are biosynthetically quiescent, however, when stimulated by extracellular signals they secrete ECM macromolecules, growth factors, and an array of cytokines. They play a critical role in maintaining homeostasis of the microenvironment and play a critical role in wound healing. During the wound healing process, growth factors released by inflammatory cells stimulate fibroblasts to migrate toward a wound where they proliferate and secrete a collagen-rich extracellular matrix. They differentiate into myofibroblasts and actively close the wound by contraction. Clone TE-7 is a reliable marker for mesodermally derived cells in various fetal and adult tissues and fibrosarcoma. It is also useful for the assessment of thymic epithelial culture contamination with fibroblasts. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goodpaster, T., et al. (2008). J. Histochem. Cytochem. 56(4): 347-358; Haynes, BF., et al. (1984). J. Exp. Med. 159(4); 1149-1168).
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | TE-7, recombinant monoclonal |
| 描述 | recombinant, expressed in HEK 293 cells |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 纯化方式 | using protein G |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry. |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| 同位素/亚型 | IgG1 |
| 表位序列 | Unknown |
| Protein ID登记号 | Unknown |
| UniProt登记号 | Unknown |
| 环保替代半岛bd体育手机客户端 分类 | Aligned,Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
