半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 6C19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Fibrillin-1. It targets an epitope within the N-terminal half.
免疫原
GST/His-tagged recombinant fragment corresponding to 95 amino acids from the N-terminal half of human Fibrillin-1.
应用
Quality Control Testing
Western Blotting Analysis: A 1:1000 dilution of this antibody detected GST/His-tagged recombinant human Fibrillin-1.Tested applicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrillin-1 in human placenta tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Fibrillin 1 recombinant fragment with a KD of 1.7 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Fibrillin-1, clone 6C19 ZooMAb, Cat. No. ZRB1019, is a recombinant Rabbit monoclonal antibody that specifically targets Fibrillin-1 and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
Fibrillin-1 (UniProt: P35555; also known as FBN1) is encoded by the FBN1 (also known as FBN) gene (Gene ID: 2200) in human. Fibrillin-1 is a structural component of the 10-12 nm diameter microfibrils of the extracellular matrix that conveys both structural and regulatory properties to load-bearing connective tissues. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Fibrillin-1 is synthesized with a signal peptide (aa 1-24) and a propeptide (aa 25-44) that are cleaved off to generate the mature Fibrillin-1, which can be further cleaved by furin between residues 2731 and 2732 to produce asprosin (aa 2732-2871). Asprosin serves as a hormone that targets the liver to increase plasma glucose levels. It promotes glucose release from hepatocytes by activating protein kinase A. Fibrillin-1 is reported to play a role in tissue homeostasis through specific interactions with growth factors, such as the bone morphogenetic proteins (BMP), growth and differentiation factors, and latent TGF-beta binding proteins. It is also reported to regulate osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels. It is also shown to negatively regulate osteoclastogenesis by binding and sequestering an osteoclast differentiation and activation factor TNFSF11. Mutations in FBN1 gene are known to cause Marfan syndrome, a disorder of the connective tissue that affects skeletal, ocular, and cardiovascular systems. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| 克隆 | recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 312.3 kDa observed mol wt ~ 35 kDa |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
