半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 1J8 is a ZooMAb?rabbit recombinant monoclonal antibody that specifically detects EZH Inhibitory protein (EZHIP). It targets an epitope within 18 amino acids from the N-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human EZH Inhibitory Protein (EZHIP).
应用
Anti-EZHIP/CXorf67, clone 1J8 ZooMAb?, Cat. No. ZRB1377, is a recombinant Rabbit monoclonal antibody that specifically detects EZHIP/CXorf67 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in lysate from HEK293 cells transfected with EZHIP.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected EZHIP/CXorf67 in lysate from HEK293 cells stably transfected with N-terminal FLAG-tagged human EZHIP.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected EZHIP/CXorf67 in human testis tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected EZHIP/CXorf67 in U2OS cell line.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
EZH inhibitory protein (UniProt: Q86X51; also known as EZHIP) is encoded by the EZHIP (also known as CXorf67) gene (Gene ID: 340602) in human. EZHIP is expressed predominantly in the gonads. In testis, it is detected in male germ cells inside the seminiferous tubules, especially in spermatogonia and round spermatids. In the ovary, it is expressed in primordial follicles and oocytes but not the external follicle cells. It is shown to inhibit PRC2/EED-EZH1 and PRC2/EED-EZH2 complex function by inhibiting EZH1/EZH2 methyltransferase activity, thereby causing down-regulation of histone H3 trimethylation on lysine 27 (H3K27me3). It inhibits H3K27me3 deposition during spermatogenesis and oogenesis. Its inhibitory effect on methyltransferase activity is attributed to its ability to limit the stimulatory effect of cofactors such as AEBP2 and JARID2. Amino acid sequence 403-423 is reported to be essential and sufficient for inhibition of PRC2/EED-EZH1 and PRC2/EED-EZH2 complex activity and the sequence 401-409 is sufficient for its interaction with EZH2. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ragazzini, R., et al. (2019). Nat. Commun. 10(1); 3858).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1J8, recombinant monoclonal |
| 描述 | recombinant, expressed in HEK 293 cells |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 51.89 kDa observed mol wt ~52 kDa |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 表位序列 | N-terminal half |
| Protein ID登记号 | NP_981952 |
| UniProt登记号 | Q86X51 |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
