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位置: 首页> 品牌> Sigma-Aldrich> Anti-Erythropoietin Antibody, clone 6E5, ZooMAb?Rabbit Monoclonal

Anti-Erythropoietin Antibody, clone 6E5, ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1366
规格纯度
recombinant, expressed in HEK 293 cells
价格
2001.06 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-Erythropoietin Antibody, clone 6E5, ZooMAb ?Rabbit Monoclonal
EPO
Epoetin
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 6E5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Erythropoietin.

免疫原

His-tagged full-length mature recombinant human Erythropoietin (without the signal peptide).

应用

Anti-Erythropoietin, clone 6E5 ZooMAb, Cat. No. ZRB1366, a recombinant Rabbit Monoclonal Antibody that targets Erythropoietin and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Erythropoietin in HUVEC and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Erythropoietin in human kidney and rat kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Erythropoietin (UniProt: P01588; also known as Epoetin, EPO) is encoded the EPO gene (Gene ID: 2056) in human. EPO is an acidic glycoprotein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off in the endoplasmic reticulum and the mature protein undergoes glycosylation in the Golgi prior to its release. EPO is mainly produced by peritubular fibroblasts in the renal cortex and to some extent by the liver in adult mammals. In fetal and neonatal mammals, it produced in the liver. EPO is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia. It is induced by ischemic preconditioning by activation of (HIF)-1alpha. It is reported to contribute to angiogenesis in response to ischemia through upregulation of VEGF/VEGFR. It binds to EPO receptor that leads to receptor dimerization and JAK2 activation that results in activation of specific downstream effectors, including STAT1 and STAT3. Endogenous EPO consists of three N-linked polysaccharide groups and one O-linked group. Although its glycosylation slightly reduces its biological activity but is shown to be essential for ensuring its prolonged circulation in the plasma. The N-glycans in its structure protect it from the action of proteases and in the modulation of its receptor binding affinity. Mutations in EPO gene can cause microvascular complications in type II diabetic subjects and erythrocytosis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bunn, HF (2013). Cold Spring Harb. Perspect. Med. 3(3); a011619).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 rabbit (recombinant)
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 6E5, monoclonal
recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 21.31 kDa
observed mol wt ~20 kDa
species reactivity rat, mouse, human
包装 pkg of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型 IgG
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1

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