半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 1J9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Cyclin D1. It targets an epitope within 19 amino acids from the C-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Cyclin D1.
应用
Immunohistochemistry (Paraffin) Analysis: A 1:10 dilution from a representative lot detected Cyclin D1 in human pancreatic cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cyclin D1 in HeLa, A431, HUVEC, MCF-7, SK-MEL-28 and HT-29 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-Cyclin D1, clone 1J9 ZooMAb, Cat. No. ZRB1313, is a recombinant rabbit monoclonal antibody that specifically detects Cyclin D1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
G1/S-specific cyclin-D1 (UniProt: P24385; also known as B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene) is encoded by the CCND1 (also known as BCL1, PRAD1) gene (Gene ID: 595) in human. Cyclin D1 is an considered to be an essential regulator of the G1-S transition in response to growth factor stimulation in somatic cells. Together with cyclin dependent kinases 4 and 6 (CDK4 and CDK6), it forms active complexes that are shown to promote cell cycle progression by phosphorylating and inactivating the retinoblastoma protein (RB). Phosphorylation of RB1 allows dissociation of E2F from the RB/E2F complex and the subsequent transcription of E2F target genes that are responsible for the progression through the G1 phase. Cyclin D1 levels are reported to rise early in G1 and continue to accumulate until the G1/S-phase boundary when its levels rapidly decline. It exhibits a short half-life of about 24 minutes and is readily degraded in the 26S proteasome. Cyclin D1 is shown to repress DNA replication by binding to proliferating cell nuclear antigen (PCNA) and Cdk2. The binding of cyclin D1 to PCNA directly inhibits DNA synthesis. The overexpression of cyclin D1 and its deregulated degradation have been linked to the development and progression of various cancers. A chromosomal aberration involving CCND1 gene is reported to cause B-lymphocytic malignancy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Alao, JP (2007). Mol. Cancer 6: 24; Albero, R., et al. (2018). J. Clin. Invest. 128(9); 4132-4147).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit (recombinant) |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1J9, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 33.73 kDa observed mol wt ~38 kDa |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| 包装 | pkg of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
