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位置: 首页> 品牌> Sigma-Aldrich> Anti-CXCR4 Antibody, clone 2L2, ZooMAb?Rabbit Monoclonal

Anti-CXCR4 Antibody, clone 2L2, ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1357
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-CXCR4 Antibody, clone 2L2, ZooMAb ?Rabbit Monoclonal
LPS-associated protein 3
CD184
Fusin
LESTR
Leukocyte-derived seven transmembrane domain receptor
Stromal cell-derived factor 1 receptor
LAP-3
SDF-1 receptor
HM89
Lipopolysaccharide-associated protein 3
LCR1
CXC-R4
C-X-C chemokine receptor type 4
FB22
NPYRL
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2L2 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human C-X-C chemokine receptor 4 (CXCR4). It targets an epitope within 12 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human C-X-C chemokine receptor type 4 (CXCR4).

应用

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CXCR4 in human adrenal gland tissue sections.
ELISA Analysis: A representative lot specifically detected CXCR4 peptide in ELISA application, but not peptides from five other proteins.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CXCR4 in Jurkat and K562 cell lines.
Affinity Binding Assay: A representative lot of this antibody bound CXCR4 with a KD of 3.6 x 10-10 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-CXCR4, clone 2L2 ZooMAb, Cat. No. ZRB1357, a recombinant Rabbit monoclonal antibody that targets human CXCR4 and is tested in Affinity Binding Assay, ELISA, Immunocytochemistry, and Immunohistochemistry (Paraffin).

目标描述

C-X-C chemokine receptor type 4 (UniProt: P61073; also known as CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-1 receptor, CD184) is encoded by the CXCR4 gene (Gene ID: 7852) in human. CXCR-4 is a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium levels and enhancing MAPK1/MAPK3 activation. CXCR-4 is expressed in numerous tissues and two isoforms have been reported that are generated by alternative splicing. Isoform 1 is the predominant form in all tissues tested. CXCR-4 is rapidly phosphorylated on serine and threonine residues in the C-terminal region upon agonist stimulation. Phosphorylation at serine 324 and serine 325 leads to recruitment of ITCH (E3 ubiquitin-protein ligase), ubiquitination and protein degradation. Sulfation on tyrosine 21 is shown to be essential for efficient binding of CXCL12/SDF-1alpha and it promotes its dimerization. Tyrosine 7 and tyrosine 12 are sulfated in a sequential manner following complete sulfation of tyrosine 21. CXCR-4 acts as a co-receptor for human HIV-1 virus isolates (CD4 being the primary receptor) and as a primary receptor for HIV-2 isolates. CXCR-4 can also bind bacterial lipopolysaccharide (LPS) and mediate LPS-induced inflammatory response, including TNF-alpha secretion by monocytes. CXCR-4 is reported to be essential for metastatic spread to organs where CXCL12 is expressed, which allows tumor cells to access cellular niches that favor tumor-cell survival and growth. Mutations in CXCR4 gene are reported to cause WHIM syndrome that is characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Burger, JA., and Kipps, TJ (2006). Blood 107(5);1761-1767).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 rabbit (recombinant)
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 2L2, monoclonal
recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt ~39.75 kDa
species reactivity human
包装 pkg of 25 μL
环保替代半岛bd体育手机客户端 特性 Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
同位素/亚型 IgG
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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