半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 2K16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Choline Acetyltransferase. It targets an epitope with in 19 amino acids from the N-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Choline acetyltransferase.
应用
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in mouse brain tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in rat brain tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in mouse brain tissue sections.
Anti-Choline Acetyltransferase (ChAT), clone 2K16 ZooMAb, Cat. No. ZRB1012, is a recombinant Rabbit Monoclonal Antibody that specifically targets Choline Acetyltransferase (aka ChAT) and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
目标描述
Choline O-acetyltransferase (UniProt: P28329; also known as EC: 2.3.1.6, CHOACTase, ChAT, Choline acetylase) is encoded by the CHAT gene (Gene ID: 1103) in human. Choline acetyltransferase a single-strand globular protein that catalyzes the biosynthesis of acetylcholine from acetyl CoA and choline. It is one of the most specific indicator for monitoring the functional state of cholinergic neurons in the central and peripheral nervous systems. It is synthesized in the perikaryon of cholinergic neurons and transported to the nerve terminals. Mutations in CHAT gene are known to cause Myasthenic syndrome that is characterized by failure of neuromuscular transmission, which include pre-synaptic, synaptic, and post-synaptic disorders. Three isoforms of Choline acetyltransferase have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oda, Y et al., (1999). Pathol. Int. 49(11); 921-937).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit (recombinant) |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 2K16, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 82.54 kDa observed mol wt ~65 kDa |
| species reactivity | mouse, rat |
| species reactivity (predicted by homology) | human |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry. |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | immunofluorescence: suitable using 1:100 immunohistochemistry: suitable using 1:100 western blot: suitable using 1:1,000 |
| 同位素/亚型 | IgG |
| UniProt登记号 | P28329 |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
| Gene Information | human ... CHAT(1103) |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
