半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 3N22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Signal transducer 24 (CD24).
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Signal transducer 24 (CD24).
应用
Anti-CD24, clone 3N22 ZooMAb, Cat. No. ZRB1564, is a recombinant Rabbit monoclonal antibody that specifically detects CD24 and is tested for use in Affinity Binding Assay, Flow Cytometry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD24 in lymphocytes, Jurkat, and Daudi cell lysates.
Flow Cytometry Analysis: 1 μg from a representative lot detected CD24 in one million Raji cells.
Affinity Binding Assay: A representative lot of this antibody bound CD24 peptide with a KD of 3.4 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Signal transducer CD24 (UniProt: P25063; also known as Small cell lung carcinoma cluster 4 antigen, CD24) is encoded by the CD24 (also known as CD24A) gene (Gene ID: 100133941) in human. CD24 is a GPI-anchor membrane glycoprotein protein that is expressed on hematopoietic cells including B cells, T cells, neutrophils, macrophages and in neuronal and ganglion cells. Its expression is also observed in many types of cancer cells. Its expression is reported to be higher levels in progenitor cells and metabolically active cells compared to terminally differentiated cells. In B cells its expression is lost when primary B-cells are induced to differentiate in antibody-forming cells. Deficiency of CD24 can result in a reduction in late pre-B and immature B-cell populations in the bone marrow. CD24 is extensively used as a marker for the differentiation of hematopoietic and neuronal cells. As a glycosyl phosphatidylinositol-anchor molecule, it mediates signal transduction by recruiting Src family protein tyrosine kinases via membrane rafts and also activates the mitogen-activated protein kinase pathway. CD24 is synthesized with a signal peptide (aa 1-26) and a propeptide (aa 60-80) that are cleaved off to produce the mature form (aa 27-59). Mutation is CD24 gene are linked to development of multiple sclerosis, a demyelinating disease of the central nervous system. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fang, X., et al. (2010). Cell. Mol. Immunol. 7(2); 100-103).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 3N22, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 8.08 kDa observed mol wt ~38 kDa |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable flow cytometry: suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
