Anti-Calretinin Antibody, clone 3K22, ZooMAb^?Rabbit Monoclonal

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 3K22 is a ZooMAb rabbit recombinant monoclonal antibody that detects Calretinin. It targets an epitope with in the internal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 99 amino acids from the internal region of rat Calretinin.

应用

Western Blotting Analysis: A 1:1000 dilution from a representative lot detected Calretinin in rat and human brain tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calretinin in PC12 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Calretinin in human cerebral cortex tissue sections.
Anti-Calretinin, clone 3K22, ZooMAb, Cat. No. ZRB5054, is a recombinant rabbit monoclonal antibody that detects Calretinin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Calretinin (UniProt: P47728; also known as CR) is encoded by the Calb2 gene (Gene ID: 117059) in rat. Calretinin belongs to the calbindin protein family and acts as a calcium-binding protein. It is expressed in both central and peripheral nervous system and is found abundantly in auditory neurons. It is involved in regulating cytosolic calcium ion concentrations and has six calcium-binding domains known as EF-hands. Its expression is shown to be regulated at the level of transcription by nuclear respiratory factor 1 (NRF-1) and E2F2 transcription factors. In rat striatum calretinin neurons form a distinct subset of medium-sized (7- 20 μm), aspiny, GABAergic interneurons. Calretinin can also serve as an important differential diagnostic tool for cystic and solid ameloblastic tumors. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Petryszyn, S., et al. (2017). J. Neural Transm. doi: 10.1007/s00702-017-1687; Kresoja-Rakic, J., et al. (2017). Front. Genet. doi: 10.3389/fgene.2017.00070).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源	rabbit (recombinant)
重组	expressed in HEK 293 cells
偶联物	unconjugated
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	3K22, monoclonal recombinant monoclonal
半岛bd体育手机客户端 线	ZooMAb?learn more
形式	lyophilized
分子量	calculated mol wt 31.4 kDa observed mol wt ~30 kDa
species reactivity	mouse, rat, human
包装	antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry.
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
technique(s)	immunocytochemistry: suitable using 1:100 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400 western blot: suitable using 1:1,000
同位素/亚型	IgG
UniProt登记号	P47728
环保替代半岛bd体育手机客户端 分类	Aligned
运输	ambient
储存温度	2-8℃
Gene Information	rat ... Calb2(117059)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1