半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 8G18 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects human CCAAT/enhancer-binding protein beta (C/EBP beta). It targets an epitope with in the N-terminal region.
免疫原
His-tagged recombinant fragment corresponding to 150 amino acids from the N-terminal half of human CCAAT/enhancer-binding protein beta (C/EBP beta).
应用
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected C-EBP beta in HeLa, A431, HUVEC, NIH 3T3 and HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected C-EBP beta in human colon and mouse colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-C-EBP beta, clone 8G18 ZooMAb , Cat. No. ZRB1105, is a highly specific rabbit recombinant monoclonal antibody that targets C-EBP beta and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
CCAAT/enhancer-binding protein beta (UniProt: P17676; also known as C/EBP beta, Liver activator protein, LAP, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, Transcription factor 5, TCF-5) is encoded by the CEBPB (also known as TCF5) gene (Gene ID: 1051) in human. C/EBP beta is a highly conserved B-Zip family member that acts as a transcription factor regulating the expression of genes involved in immune and inflammatory responses. It is expressed at low levels in the lung, kidney and spleen and is induced by endoplasmic reticulum stress. It can bind to DNA either as a homodimer or a heterodimer with ATF4. C/EBP beta plays a significant role in adipogenesis, as well as in the gluconeogenesis, liver regeneration, and hematopoiesis. During adipogenesis, it is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes giving rise to the adipocyte phenotype. It has a pro-mitotic effect on many cell types, including hepatocytes and adipocytes, but has an anti-proliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. C/EBP beta is phosphorylated at threonine 235 by MAPK and CDK2 that serves to prime phosphorylation at threonine 226 and serine 231 by GSK3beta and acquire DNA-binding as well as transactivation activities that are required to induce adipogenesis. However, O-glycosylation at serine 227 and serine 228 is shown to prevent phosphorylation on threonine 235, serine 231, and threonine 226 and reduces its DNA binding activity that delays adipocyte differentiation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit (recombinant) |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 8G18, recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 36.11 kDa observed mol wt ~42 kDa |
| species reactivity | human, mouse |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable (peptide) |
| 同位素/亚型 | IgG |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
