半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone F6 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects BMI-I. It targets an epitope within the N-terminal region.
免疫原
Recombinant fragment corresponding to the first 202 amino acids from Mouse Polycomb complex protein BMI-1.
应用
Anti-BMI-1, clone F6 ZooMAb, Cat. No. ZMS05637, is a recombinant mouse monoclonal antibody that specifically targets BMI-1 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BMI-1 in U2OS cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected BMI-1 in human placenta tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected BMI-1 in HeLa, A431, HUVEC and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Polycomb complex protein BMI-1 (UniProt: P35226; also known as Polycomb group RING finger protein 4, RING finger protein 51) is encoded by the BMI1 (also known as PCGF4, RNF51) gene (Gene ID: 100532731) in human. BMI-I (B cell-specific Moloney murine leukemia virus integration site 1) is a component of the polycomb repressive complex 1 (PRC1) that is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. This complex acts via chromatin remodeling and modification of histones and mediates mono-ubiquitination of histone H2A lysine 119, rendering chromatin. The canonical PRC1 complex consists of four core subunits: CBX (polycomb; CBX2/4/6/7/8), PCGF (polycomb group factors; PCGF1 6), PHC (polyhomeotic homologues; PHC1/2/3), and RING E3 ligase (RING1A/B). BMI-I has three domains. The N-terminal RING domain, a central domain, and a C-terminal proline-rich domain. The RING domain forms a complex with RINGA/B proteins that constitutes the heterodimeric E3 ubiquitin ligase subunit of the PRC1 complex. The central is a ubiquitin-like (UBL) domain and is involved in protein-protein interactions and its binding partners include the polyhomeotic proteins (PHC1, PHC2, PHC3). The central domain of BMI-I is reported to be essential for its oncogenic activity and is shown to be essential for transcriptional repression activity. Higher expression of BMI1 gene is overexpressed in certain mantle cell lymphomas and several other cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gray, F., et al. (2016). Nat. Commun. 7; 13343).
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse (recombinant) |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | F6, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 36.95 kDa observed mol wt ~38 kDa |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | rabbit, rat |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
