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位置: 首页> 品牌> Sigma-Aldrich> Anti-β-Tubulin III Antibody, clone 5H16, ZooMAb?Rabbit Monoclonal

Anti-β-Tubulin III Antibody, clone 5H16, ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1140
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-β-Tubulin III Antibody, clone 5H16, ZooMAb ?Rabbit Monoclonal
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 5H16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects beta-Tubulin III. It targets an epitope with in 10 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region of human beta-Tubulin III chain.

应用

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected -Tubulin III in human cerebral cortex and mouse cerebellum tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected -Tubulin III in SH-SY5Y cells.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Anti-beta-Tubulin III, clone 5H16 ZooMAb, Cat. No. ZRB1140-25UL, is a highly specific rabbit recombinant monoclonal antibody that targets beta-Tubulin III and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Tubulin beta-3 chain (UniProt: Q13509; also known as Tubulin beta-4 chain, Tubulin beta-III, beta-Tubulin 3) is encoded by the TUBB3 (also known as TUBB4) gene (Gene ID: 10381) in human. Tubulin is one of several members of a small family of globular proteins, which polymerize to form microtubules. The most common members of the tubulin family are alpha-tubulin and beta-tubulin that assemble on to microtubules forming large polymers. Tubulin binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. GTP-binding site of beta-tubulin III is localized to amino acids 140-146. Beta-III tubulin is a unique microtubule subunit that is expressed exclusively in neurons, but its higher expression is also observed in most cancerous tissues. Due to it is unique expression pattern it is often utilized as a marker to identify neuronal cells from other brain cells such as glia. Beta-tubulin III plays a critical role in proper axon guidance and maintenance. Beta-tubulin III undergoes phosphorylation on serine 172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase and this phosphorylation inhibits tubulin incorporation into microtubules. Increased expression of beta-tubulin III been shown to correlate with resistance to anti-microtubule agents. Mutations in TUBB3 gene have been linked to congenital fibrosis of extraocular muscles that causes ocular motility disorder affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 rabbit (recombinant)
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 5H16, recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 50.43 kDa
observed mol wt ~52 kDa
species reactivity mouse, rat, human
包装 antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)
同位素/亚型 IgG
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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