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位置: 首页> 品牌> Sigma-Aldrich> Anti-Rab7A Antibody, clone 4E15 ZooMAb?Rabbit Monoclonal

Anti-Rab7A Antibody, clone 4E15 ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1120
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-Rab7A Antibody, clone 4E15 ZooMAb ?Rabbit Monoclonal
Ras-related protein Rab-7A
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.

特异性

Clone 4E15 is a Rabbit recombinant monoclonal antibody that specifically detects Rab7A. It targets an epitope within 17 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Rab7A.

应用

Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Rab7A in human kidney tissue sections.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rab7A in Cos-7 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Rab7A with a KD of 2.2 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Rab7A in HeLa, A431, HepG2 and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Ras-related protein Rab-7A (UniProt: P51149; also known as Rab-7a) is encoded by the RAB7A (also known as RAB7) gene (Gene ID: 7879) in human. Rab7A is a highly conserved member of the large superfamily of Ras-like GTPases. It is widely expressed, peripheral membrane protein that is localized mainly to late endosomes and regulates transport from early endosomes to late endosomes and from late endosomes to lysosomes. It is not only important for vesicle formation, transport and fusion but also for cargo selection and sorting. Like other GTPases, Rab7 also undergoes characteristic cyclical mechanism of activation and inactivation depending on GTP binding and hydrolysis. It also plays a many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transporter mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. It also participates in some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes and autophagosomes. More recently, it is reported to be essential for vesicular trafficking and cell surface expression of ACE2 and loss of Rab7A is reduces SARS-CoV-2 entry into cells by sequestering the ACE2 receptor inside cells through altered endosomal trafficking. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Daniloski, Z., et al. (2021). Cell. 184(1); 92-105; Guerra, F., and Bucci, C. (2016). Cells. 5(3); 34).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 rabbit
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 4E15, recombinant monoclonal
描述 recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 23.49 kDa
observed mol wt ~30 kDa
纯化方式 using Protein A
species reactivity human, monkey, mouse
包装 antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about thePrinciples of Green Chemistry.
增强验证 recombinant expression
Learn more aboutAntibody Enhanced Validation
technique(s) affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型 IgG
表位序列 C-terminal
Protein ID登记号 NP_004628.4?
UniProt登记号 P51149
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 13 - Non Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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