半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 6F13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Phospholipase D2. It targets an epitope within 21 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Phospholipase D2.
应用
Anti-PLD2, clone 6F13 ZooMAb, Cat. No. ZRB1629, is a recombinant Rabbit monoclonal antibody that detects Phospholipase D2 and is tested in Affinity Binding Assay, Flow Cytometry and Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Quality Control Testing
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PLD2 in NIH3T3 cell lysate.Tested applicationsImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PLD2 in HeLa, A431, and d NIH 3T3 cells..Flow Cytometry Analysis: 1 μg from a representative lot detected PLD2 in one million NIH3T3 cells.Affinity Binding Assay: A representative lot of this antibody bound PLD2 peptide with a KD of 1.7 x 10-6 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PLD2 in human pancreas and human kidney tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Phospholipase D2 (UniProt: O14939; also known as EC:3.1.4.4, PLD 2, hPLD2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2) is encoded by the PLD2 gene (Gene ID: 5338) in human. PLD2 is a peripheral membrane protein that displays ubiquitous expression and plays a role in cell membrane lipid reorganization and serves as a key cell signaling protein. It is activated by a wide variety of G-protein coupled receptors and receptor tyrosine kinases in response to a wide variety of agonists. It hydrolyzes the membrane phospholipid phosphatidylcholine to choline and the lipid messenger phosphatidic acid (PA). PA can act as a central lipid second messenger for various signaling pathways and participates in many physiological processes, including cell proliferation, migration, and cytoskeletal organization. PA is also involved in the activation of Ras‐GTPase by recruiting its exchange factor Sos or Rac1to the plasma membrane. PLD2 is reported to positively regulate proliferation of CD8+ T cells that play a critical role in cancer cell elimination and suppression of tumor progression. PLD2 is reported to degrade HIF-1 and thereby reduces VEGF secretion by endothelial cells that results in reduction of tumor neovascularization and growth. PLD2 has PX and PH domains at its regulatory N‐ erminus that are putatively responsible for its GEF activity. It also has two conserved HKD domains are responsible for the lipase activity and conversion of PC into PA. Its catalytic region is localized within amino acids 441-788. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Bich, VNT., et al. (2018). Sci. Rep. 8; 6283; Frohman, MA. (2015). Trends Pharmacol. Sci. 36(3); 137-144; Park, MH., et al. (2015). Exp. Mol. Med. 47; 196; Gomez-Cambronero, J. (2012). J. Leukocyte Biol. 92(3); 461-467).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 200 |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
克隆 | recombinant monoclonal |
半岛bd体育手机客户端 线 | ZooMAb?learn more |
形式 | lyophilized |
分子量 | calculated mol wt 105.98 kDa observed mol wt ~ 105 kDa |
species reactivity | mouse, human |
包装 | antibody small pack of 25 μL |
环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | affinity binding assay: suitable flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG |
环保替代半岛bd体育手机客户端 分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich