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位置: 首页> 品牌> Sigma-Aldrich> Anti-Phospho-IRF-3-(Ser396) Antibody, clone 2M20 ZooMAb?Rabbit Monoclonal

Anti-Phospho-IRF-3-(Ser396) Antibody, clone 2M20 ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1568
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-Phospho-IRF-3-(Ser396) Antibody, clone 2M20 ZooMAb ?Rabbit Monoclonal
Interferon regulatory factor 3
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.

特异性

Clone 2M20 is a Rabbit recombinant monoclonal antibody that specifically detects Interferon regulatory factor 3 (IRF-3) phosphorylated on serine 396.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids surrounding phosphoserine 396 from the C-terminal region of human Interferon regulatory factor 3 (IRF-3).

应用

Quality Control Testing
Evaluated by Western Blotting in lysate from HeLa cells overnight serum starved and treated with Calyculin A and Okadaic acid.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected IRF-3 phosphorylated on serine 396 in lysates from HeLa cells overnight serum starved and treated with Calyculin A (50 nM) and Okadaic acid (500 nM).
Tested applications
Affinity Binding Assay:A representative lot of this antibody bound Phosphorylated IRF3-Ser396 Peptides with a KD of 4.7 x 10-7 in an affinity binding assay.
Peptide Inhibition Assay Analysis: Target band detection in lysate from HeLa cells treated with Calyculin A and Okadaic acid was prevented by preblocking of a representative lot with phosphorylated IRF3-Ser396 peptide.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected IRF-3 in human colon cancer tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Interferon regulatory factor 3 (UniProt: Q14653; also known as IRF-3) is encoded by the IRF3 gene (Gene ID: 3661) in human. IRF-3 is a transcription factor that is constitutively expressed in a variety of tissues and plays a role in intracellular immune response against DNA and RNA viruses. It regulates the transcription of type I interferon genes (IFN-a and IFN-b) and interferon-stimulated genes by binding to an interferon-stimulated response element in their promoters. IRF-3 contains a DNA-binding region (aa 5-111), nuclear export signal (aa 139-149), IRF-interacting domain, and a C-terminal serine-rich region that contains several phosphorylation sites. It shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. In the activated state, IRF3 interaction with CREB Binding Protein prevents its export to the cytoplasm. Although some of these serines are shown to be phosphorylated in the resting state, further phosphorylation of specific residues is required for its activation and nuclear translocation. In uninfected cells, IRF-3 is present in the cytoplasm, however, following infection, it undergoes phosphorylation on serine 385, serine 386, threonine 390, and serine 396. These phosphorylation events induce a conformational change that leads to its dimerization and nuclear localization where it associates with CREB binding protein to form dsRNA activated factor 1 that subsequently leads to the activation of type I interferons and ISG genes. Phosphorylation and subsequent activation of IRF-3 shown to be inhibited by vaccinia virus protein E3. Mutations in IRF3 gene have been linked to infection-induced, Herpes-specific acute encephalopathy that is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Honda, K., et al. (2006). Immunity. 25(3); 349-360; Kumar, KP., et al. (2000). Mol. Cell Bol. 20(11); 4159-4168).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 rabbit
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 2M20, recombinant monoclonal
描述 recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 47.22 kDa
observed mol wt ~55 kDa
纯化方式 using Protein A
species reactivity human
species reactivity (predicted by homology) monkey, horse, bovine
包装 antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about thePrinciples of Green Chemistry.
增强验证 recombinant expression
Learn more aboutAntibody Enhanced Validation
technique(s) affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型 IgG
表位序列 C-terminal
Protein ID登记号 NP_001562
UniProt登记号 Q14653
application(s) peptide synthesis
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 13 - Non Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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