Anti-PDK4 Antibody, clone 2H5 ZooMAb^?Rabbit Monoclonal

半岛bd体育手机客户端 说明

一般描述

ZooMAb^?antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^?antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2H5 is a ZooMAb^?rabbit recombinant monoclonal antibody that specifically detects Pyruvate dehydrogenase kinase isoform 4 (PDK4). It targets an epitope within 18 amino acids from the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Pyruvate dehydrogenase kinase isoform 4 (PDK4).

应用

Quality Control Testing
Evaluated by Western Blotting in L6 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PDK4 in L6 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PDK4 in human skeletal muscle tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PDK4 in human heart tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound PDK4 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PDK4 in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PDK4, clone 2H5 ZooMAb^?, Cat. No. ZRB1921, is a recombinant Rabbit monoclonal antibody that specifically targets PDK4 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial (UniProt: Q16654; also known as EC:2.7.11.2, Pyruvate dehydrogenase kinase isoform 4, PDK4) is encoded by the PDK4 (also known as PDHK4) gene (Gene ID: 5166) in human. PDK4 is a homodimeric mitochondrial matrix enzyme that is ubiquitous in its distribution, but higher levels of expression are found in heart and skeletal muscle. It plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid (TCA) cycle and down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase is shown to reduce glucose utilization and increase fat metabolism in response to prolonged fasting and starvation. PDK4 plays a significant role in maintaining normal blood glucose levels under conditions of starvation. By regulating pyruvate dehydrogenase activity, it plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, it mediates cellular responses to glucose levels and to a high-fat diet. This ZooMAb^?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
生物来源	rabbit
重组	expressed in HEK 293 cells
偶联物	unconjugated
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	2H5, recombinant monoclonal
描述	recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线	ZooMAb?learn more
形式	lyophilized
分子量	calculated mol wt 46.47 kDa observed mol wt ~52 kDa
species reactivity	human, rat
species reactivity (predicted by homology)	monkey, bovine
包装	antibody small pack of 25 μL
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
technique(s)	affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable
同位素/亚型	IgG
表位序列	N-terminal half
Protein ID登记号	NP_002603.1
UniProt登记号	Q16654
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1