半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 3G6 is a ZooMAb?rabbit recombinant monoclonal antibody that specifically detects PTEN. It targets an epitope surrounding phosphotyrosine 240.
免疫原
BSA-conjugated linear peptide corresponding to 17 amino acids surrounding phosphotyrosine 240 from the C-terminal half of human PTEN.
应用
Anti-p-PTEN (Tyr240), clone 3G6 ZooMAb?, Cat. No. ZRB2134, is a recombinant Rabbit monoclonal antibody that specifically detects PTEN phosphorylated on Tyr240 and is tested for use in Affinity Binding Assay, Immunohistochemistry, and Western Blotting.
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human astrocytoma tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected PTEN phosphorylated on tyrosine 240 in human astrocytoma tissue sections.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PTEN phosphorylated on tyrosine 240 in normal human brain tissue sections.
Immunohistochemistry Applications: A representative lot detected PTEN phosphorylated on tyrosine 240 in Immunohistochemistry applications (Ma, J., et. al. (2019). Cancer Cell. 35(3):504-518).
Affinity Binding Assay: A representative lot of this antibody bound phopho-PTEN (Tyr240) peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected wild-type PTEN, but not the mutant PTEN.
Western Blotting Analysis: A representative lot detected PTEN phosphorylated on tyrosine 240 in Western Blotting applications (Ma, J., et. al. (2019). Cancer Cell. 35(3):504-518).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (UniProt: P60484; also known as Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog) is encoded by the PTEN (also known as MMAC1, TEP1) gene (Gene ID: 5728) in human. PTEN belongs to the family of lipid phosphatases. It contains an N-terminal phosphatase catalytic domain, a C2-domain that binds to phospholipid membranes, and a C-terminal regulatory tail. It is a dual-specific phosphatase, which dephosphorylates both lipid and protein substrates. PTEN suppresses the activity of a major cell proliferation pathway in the cytoplasm; as such, its absence or functional deficiency is associated with tumor growth. PTEN blocks the phosphatidylinositol 3-kinase/Akt signaling pathway by removing the phosphate in the 3-position on phosphoinositide-3, 4, 5-trisphosphate (PIP3). Dephosphorylation of PIP3 is an important factor in cell growth, proliferation, apoptosis, and survival. Dephosphorylation of PIP3 blocks Akt signaling that can lead to higher activity of pro-apoptotic molecules such as Bad and Caspase-9. PTEN activity, stability, localization, and conformation are controlled through complex regulatory mechanisms, including the reversible phosphorylation of several Serine/Threonine residues in its unfolded C-terminal tail region (PTEN-C-tail, residues 351-403). Alternative translation of PTEN is reported to encode a 576-amino acid translational variant that is known as PTEN-Long with a 173-amino acid domain at its N-terminus followed by the classical 403 amino acids of PTEN. PTEN-Long is a membrane permeable, secreted protein that can enter other cells and acts as an antagonist of the PI3K pathway. PTEN is considered a major tumor suppressor and its mutations are reported in several human tumors. Over seventy different types of PTEN mutation have been recorded in patients with Cowden syndrome. PTEN -/- mice exhibit higher levels of 3 -phosphorylated phospholipids and die during embryogenesis due to the failure of developmental apoptosis. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ferrarelli, LK., et al. (2019). Sci. Signal 16(577); eaax6492; Hopkins, BD., et al. (2013). Science. 341(6144); 399-402).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | rabbit |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 3G6, recombinant monoclonal |
| 描述 | recombinant, expressed in HEK 293 cells |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 47.17 kDa observed mol wt ~52 kDa |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey, rat, mouse, bovine |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 表位序列 | C-terminal half |
| Protein ID登记号 | NP_000305.3 |
| UniProt登记号 | P60484 |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
