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位置: 首页> 品牌> Sigma-Aldrich> Anti-Nucleolin Antibody, clone 5D13 ZooMAb?Rabbit Monoclonal

Anti-Nucleolin Antibody, clone 5D13 ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1415
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-Nucleolin Antibody, clone 5D13 ZooMAb ?Rabbit Monoclonal
Protein C23
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 5D13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Nucleolin. It targets an epitope within 16 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Nucleolin.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nucleolin in A431 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nucleolin in HeLa, HUVEC, as A431 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nucleolin in human small intestine tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-Nucleolin, clone 5D13 ZooMAb, Cat. No. ZRB1415, is a recombinant Rabbit monoclonal antibody that targets Nucleolin and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Nucleolin (UniProt: P19338; also known as Protein C23) is encoded by the NCL gene (Gene ID: 4691) in human. Nucleolin is a multifunctional phosphoprotein that is ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. It is one of the most abundant non-ribosomal proteins that accounts for about 10% of the protein content within the nucleolus. It is found associated with intra-nucleolar chromatin and pre-ribosomal particles. It plays important roles in various steps of ribosomal synthesis, including the transcription of rDNA repeats, the modification and processing of pre-rRNA, the assembly of pre-ribosomal particles and nuclear-cytoplasmic transport of ribosomal proteins and subunits. Nucleolin contains three structural and multifunctional domains: the N-terminal domain contains several acidic stretches; two to four RNA-binding domains (RNA recognition motifs or RRM) in the central region; and a glycine/arginine-rich domain (GAR) domain at the C-terminus. Nucleolin is shown to be highly phosphorylated. It undergoes extensive phosphorylation by casein kinase 2 at interphase and by CDC2 during mitosis. It has been reported that Nucleolin phosphorylation at interphase is correlated with active rRNA transcription. Although the mammalian Nucleolin has a predicted molecular weight of about 77 kDa, its apparent molecular weight lies between 100 and 110 kDa due to highly phosphorylated amino acids in the N-terminal domain. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tajrishi, MM et al., (2011). Commun. Integr. Biol. 4(3); 267-275).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.03 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 rabbit
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 76.61 kDa
observed mol wt ~110 kDa
species reactivity human
包装 pkg of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型 IgG
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1

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