半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 1F11 is a ZooMAb?rabbit recombinant monoclonal antibody that specifically detects Metabotropic glutamate receptor 5 (mGluR5). It targets an epitope within 23 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 23 amino acids the C-terminal region of human Metabotropic glutamate receptor 5 (mGluR5).
应用
This rabbit recombinant monoclonal Anti-mGluR5, clone 1F11 ZooMAb?Rabbit Monoclonal, Cat. No. ZRB1270 is used in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry and Affinity Binding Assay, for the detection of mGluR5.
Quality Control Testing
Evaluated by Western Blotting in Rat brain microsome.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected mGluR5 in Rat brain microsome.
Tested applications
Affinity Binding Assay: A representative lot of this antibody bound mGluR5 peptide with a KD of 2.0 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected mGluR5 in E18 rat cortical cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected mGluR5 in rat brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Metabotropic glutamate receptor 5 (UniProt: P41594; also known as mGluR5) is encoded by the GRM5 (also known as GPRC1E, MGLUR5) gene (Gene ID: 2915) in human. Glutamate receptors can be grouped into ionotropic receptors and metabotropic receptors. Excitotoxic effects of glutamate analogs are primarily achieved through the activation of the ionotropic receptors. Glutamate can also activate metabotropic receptors that couple GTP-binding proteins to intracellular second messengers. These receptors are distributed throughout the central nervous system and are located at synaptic as well as extra-synaptic sites in neurons and glia. Metabotropic receptors contain a large extracellular N-terminal domain known as the Venus flytrap domain (VFD) that contains the glutamate-binding site and has two lobes that sit one atop the other and bind glutamate in a cleft between the two lobes. The VFD dimer can exist in open-open, open-closed, or in closed-closed state. The open-open state is an inactive conformation and is stabilized by antagonists. At least eight metabotropic glutamate receptors (mGluR1-8) have been reported. They are classified into three groups based on their sequence homology, agonist pharmacology, and coupling to intracellular transduction mechanisms. The mGluR1 and mGluR5 belong to group I and these receptors are coupled to post-synaptic inositol phosphate metabolism. They couple to Gq/G11 and activate phospholipase Cβ that hydrolyzes phosphoinositides to generate inositol 1, 4, 5-trisphosphate (IP3) and diacyl- glycerol (DAG), both of which participate in the activation of protein kinase C. Activation of Group I receptors leads to depolarization and increases neuronal excitability. mGluR1 is present at high levels in the cerebellum and thalamus and mGluR5 is found in superficial cortical layers and the olfactory bulb. The activation of mGluR1 generates a single spike in Ca2+ concentrations, whereas mGluR5 activation leads to Ca2+ oscillations via phosphorylation/dephosphorylation cycles. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | rabbit |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 1F11, recombinant monoclonal |
描述 | recombinant, expressed in HEK 293 cells |
半岛bd体育手机客户端 线 | ZooMAb?learn more |
形式 | lyophilized |
分子量 | calculated mol wt 132.47 kDa observed mol wt ~155 kDa |
species reactivity | rat |
species reactivity (predicted by homology) | bovine, mouse, human |
包装 | antibody small pack of 25 μL |
增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG |
表位序列 | C-terminus |
Protein ID登记号 | NP_001137303.1 |
UniProt登记号 | P41594 |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
Sigma-Aldrich