半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 2J13 is a ZooMAb rabbit recombinant monoclonal antibody that detects human KRas.
免疫原
GST/His-tagged full-length recombinant human KRas.
应用
Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected KRas in human kidney tissue sections.
RNAi/siRNA/Gene Knockdown Analysis: A 1:1,000 dilution from a representative lot detected KRas in lysates from wild type HeLa cells, but not in lysate from KRAS knockout HeLa cells.
Anti-KRas, clone 2J13, ZooMAb, Cat. No. ZRB003845, is a recombinant Rabbit Monoclonal antibody that detects human KRas and has been tested for use in Immunohistochemistry (Paraffin), RNAi/siRNA/Gene Knockdown, and Western Blotting.
目标描述
GTPase KRas (UniProt P01116; also known as c-K-Ras, c-Ki-Ras, K-Ras 2, Ki-Ras) is encoded by the KRAS (also known as KRAS2, RASK2) gene (Gene ID 3845) in human. Ras proteins are small GTPases, which control signaling pathways for growth, proliferation, and differentiation. Mammalian genomes harbor three Ras genes (HRAS, NRAS, and KRAS) that encode four GTPases, two of which (K-Ras4A and K-Ras4B) are transcribed from the KRAS gene via two alternative fourth exons. Ras proteins share nearly identical sequence homology in sequence encoded by exons 1-3 (a.a. 1-165), while diverge in their C-terminal 24-a.a. hypervariable region (HVR) encoded by exon 4. The first 165 amino acids form the catalytic G domain that mediates GDP/GTP binding and association with effectors, exchange factors, and GTPase-activating proteins (GAPs), while the C-terminal HVR is needed for Ras membrane localization, a process necessary for Ras function. In addition to the removal of initiator methionine (Met1), Ras proteins are post-translationally modified by farnesylation of Cys186, followed by proteolytical cleavage their C-terminal end three amino acids (aa 187-189) and carboxyl methylation of the newly formed C-terminal prenylcysteine. H-Ras, N-Ras, and K-Ras4A are further palmitoylated on cysteine residue(s) near the C-terminus (Cys181/Cys184 in H-Ras, Cys181 in N- and K-Ras), while K-Ras4B lacks a palmitoylation site. Palmitoylation is required for targeting H- and N-Ras to the plasma membrane (PM), while K-Ras4A and K-Ras4B contain a series of lysines that constitute a polybasic region (PBR) capable of interacting with the inner leaflet of the PM through an electrostatic interaction. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit (recombinant) |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 2J13, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 21.66 kDa observed mol wt ~21 kDa |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about thePrinciples of Green Chemistry. |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | gene knockdown: suitable using 1:1,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400 western blot: suitable using 1:1,000 |
| 同位素/亚型 | IgG |
| UniProt登记号 | P01116 |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
| Gene Information | human ... KRAS; KRAS2; RASK2(3845) |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
Sigma-Aldrich
m.cnreagent.com
