半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1J12 is a ZooMAb?rabbit recombinant monoclonal antibody that specifically detects Krueppel-like factor 5 (KLF5). It targets an epitope within 22 amino acids from the C-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human Krueppel-like factor 6 (KLF5).
应用
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected KLF5 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected KLF5 in NIH3T3 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected KLF5 in human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound KLF5 peptide with a KD of 2.0 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected KLF5 in PC3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-KLF5, clone 1J12 ZooMAb?, Cat. No. ZRB1194, is a recombinant Rabbit monoclonal antibody that specifically targets KLF5 and is tested for use in in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
Krueppel-like factor 5 (UniProt: Q13887; also known as Basic transcription element-binding protein 2, BTE-binding protein 2, Colon krueppel-like factor, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Transcription factor BTEB2) is encoded by the KLF5 (also known as BTEB2, CKLF, IKLF) gene (Gene ID: 688) in human. KLF5 is a member of the krueppel C2H2-type zinc-finger protein family that is expressed in testis and placenta. It serves as a transcription factor that binds to GC box promoter elements and activates the transcription of several genes. It contributes to the regulation of various cellular processes, including cell proliferation, differentiation, angiogenesis, and migration. KLF5 is reported to function as an oncogenic transcription factor in several cancer types, including breast, bladder, and intestinal cancers. Its expression can be induced by various oncogenic and pro-inflammatory factors, including TNF- , lipopolysaccharides, and interleukin-1β. Co-expression of KLF5 and TNFRSF11? correlates significantly with tumorigenesis in cervical tissues where it promotes cell proliferation, migration, and invasiveness in a manner dependent partly on TNFRSF11? expression. TNF- induced KLF5 is shown to positively regulate TNFRSF11? expression by binding to the TNFRSF11? promoter. KLF5 expression is reported to be regulated by estrogen and progesterone in breast cancer cells where estrogen facilitates KLF5 degradation and progesterone induces its expression. Loss of KLF5 in uterine tissue is reported to cause infertility due to defective implantation. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ma, D., et al. (2017). Sci. Rep. 7; 15683; Sun, X., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(4); 114-1150).
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1J12, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt ~ 50.79 kDa observed mol wt ~ 60 kDa |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | bovine, monkey |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
Sigma-Aldrich
m.cnreagent.com
