半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 4E14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects GABA Transporter 3. It targets an epitope within 21 amino acids from the C-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of rat GABA Transporter 3.
应用
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GABA Transporter-3 in rat brain tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GABA Transporter-3 in PC12 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GABA Transporter-3 in rat brain tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound GABA Transporter-3 peptide with a KD of 2.4 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-GABA Transporter-3, clone 4E14 ZooMAb, Cat. No. ZRB1070, is a recombinant Rabbit monoclonal antibody that detects GABA transporter 3 and is tested for use in is used in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
Sodium- and chloride-dependent GABA transporter 3 (UniProt: P31647; also known as GAT-3, Solute carrier family 6 member 11, GABA Transporter 3) is encoded by the Slc6a11 (also known as Gabt3, Gat-3, Gat-b) gene (Gene ID: 79213) in rat. GABA is reported to be the main inhibitory neurotransmitter in the mammalian brain, particularly in the basal ganglia network. Following its release from the presynaptic terminal, it is rapidly cleared from the extracellular space by GABA transporters (GATs) resulting it s the termination of inhibitory synaptic transmission. Thus far, four different GATs have been described in literature, GAT-1, GAT-2, GAT-3, and the Betain/GABA transporter type 1. GAT-3 is a multi-pass membrane protein that has one extracellular domain (aa 147-220), two cytoplasmic domains (aa 1-53 and 567-627), and twelve helical domains. It is shown to be highly expressed in the glomerular layer of the olfactory bulb, the inner nucleus of the retina, the thalamic paraventricular nucleus, and the globus pallidus. It terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. The GABA-transporting action of GATs is dependent on the Na+ gradient across the membrane. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jin XT., et al. (2011). Front. Syst. Neurosci. 5; 63).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 4E14, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 69.95 kDa observed mol wt ~58 kDa |
| species reactivity | rat, mouse |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
