半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 3H10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Gamma-aminobutyric acid receptor subunit a1
免疫原
GST/His-tagged recombinant fragment corresponding to 125 amino acids from the N-terminal, extracellular domain of human GABA(A) receptor subunit a1 .
应用
Anti-GABA A Receptor a1, clone 3H10 ZooMAb, Cat. No. ZRB1626, is a recombinant Rabbit monoclonal antibody that specifically targets GABA A Receptor a1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GABA A Receptor 1 in SH-SY5Y cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected GABA A Receptor 1 in human cerebellum, mouse brain membrane, and rat brain tissue lysates.Affinity Binding Assay: A representative lot of this antibody bound GABA A Receptor a1 with a KD of 4.2 x 10-6 in an affinity binding assay.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GABA A Receptor 1 in PC12 and SH-SY5Y cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Gamma-aminobutyric acid receptor subunit a1 (UniProt: P14867; also known as GABA(A) receptor subunit alpha-1) is encoded by the GABRA1 gene (Gene ID: 2554) in human. GABA is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. On binding of two GABA molecules to the extracellular receptor domain, a conformational change is induced in the oligomer and Cl moves into the cell leading to an inhibitory hyperpolarization. GABAA and GABAB receptors differ in their pharmacological, electrophysiological, and biochemical properties. GABA a1 receptor subunit is part of the heteropentamer formed by a combination of a1, b2, and y2 subunits. GABA A receptor a1 subunit is synthesized with a signal peptide (aa 1-29) which is subsequently cleaved off to generate the mature form that contains four transmembrane domains (aa 252-273; 279-300; 313-334; and 422-443), one extracellular domain (aa 28-251), and a cytoplasmic domain (aa 335-421). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhu, S., et al. (2018). Nature. 559(7712); 67-72; Goetz, T., et al. (2007). Prog. Brain Res. 160; 21-41).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| 克隆 | recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 51.8 kDa observed mol wt ~ 51 kDa |
| species reactivity | mouse, human, rat |
| species reactivity (predicted by homology) | monkey, bovine |
| 包装 | antibody small pack of 25 μL |
| 环保替代半岛bd体育手机客户端 特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunocytochemistry: suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 环保替代半岛bd体育手机客户端 分类 | Aligned |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
