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位置: 首页> 品牌> Sigma-Aldrich> Anti-Gab1 Antibody, clone 4B20, ZooMAb?Rabbit Monoclonal

Anti-Gab1 Antibody, clone 4B20, ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1348
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-Gab1 Antibody, clone 4B20, ZooMAb ?Rabbit Monoclonal
GRB2-associated binder 1
GRB2-associated-binding protein 1
Growth factor receptor bound protein 2-associated protein
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 4B20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects GRB2-associated-binding protein 1 (Gab1). It targets an epitope within 30 amino acids from the C-terminal region.

免疫原

KLH-conjugated Linear Peptide corresponding to 30 amino acids from the C-terminal region of Human Gab1.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Gab1 in fetal bovine heart endothelial (FBHE) cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Gab1 in human small intestine tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Gab1 peptide with a KD of 4.0 x 10-6 in affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-Gab1, clone 4B20 ZooMAb, Cat. No. ZRB1348, is a recombinant Rabbit monoclonal antibody that specifically targets GRB2-associated-binding protein 1 (Gab1) and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

GRB2-associated-binding protein 1 (UniProt: Q13480; also known as GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1; Gab1) is encoded by the GAB1 gene (Gene ID: 2549) in human. Gab1 is a scaffolding protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. It recruits PI-3 kinase and other effector proteins in response to the activation of many receptor tyrosine kinases (RTKs). Gab1 can be phosphorylated on at least 16 sites by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta, hepatocyte growth factor/scatter factor receptor, and epidermal growth factor receptor. Phosphorylation of Gab1 on tyrosine 627 and tyrosine 659 is shown to be critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. Tyrosine phosphorylation of Gab1 mediates interaction with several proteins that contain SH2 domains. Gab1 plays important roles in embryonic development and cell migration and is shown to be a critical component in the interaction between focal adhesion kinase and Met in cancer cell invasion. Gab1 is also reported to be a major target in linoleic acid (LA)-induced enhancement of tumorigenesis. Mutations in GAB1 gene are known to cause autosomal recessive deafness that is characterized by severe to profound hearing loss resulting from damage to the neural receptors of the inner ear or the nerve pathways to the brain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mattoon, DR., et al. (2004). BMC Biol. 2; 24; Mouradian, M., et al. (2014). Prostaglandins Leukot. Essent. Fatty Acids. 90(4); 105-115).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 rabbit (recombinant)
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 4B20, monoclonal
recombinant monoclonal
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 76.62 kDa
observed mol wt ~100 kDa
species reactivity human, bovine
包装 pkg of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型 IgG
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 13 - Non Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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