半岛bd体育手机客户端 说明
一般描述
ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 1H21 is a ZooMAb?rabbit recombinant monoclonal antibody that specifically detects human T-cell surface glycoprotein CD8 beta chain (CD8β). It targets an epitope within 13 amino acids from the extracellular domain.
免疫原
KLH-conjugated linear peptide corresponding to 13 amino acids from the extracellular domain of human T-cell surface glycoprotein CD8 beta chain (CD8β).
应用
Anti-CD8β, clone 1H21 ZooMAb?, Cat. No. ZRB1255, is a recombinant Rabbit monoclonal antibody that targets CD8β and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in PBMC cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD8β in peripheral blood mononuclear cell (PBMC) lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD8β in human thymus and human spleen tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD8β in human thymus tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CD8β peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Flow Cytometry Analysis: 1 βg from a representative lot detected CD8β in one million Hut78 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD8β in Jurkat cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
T-cell surface glycoprotein CD8 beta chain (UniProt: P10966; also known as CD8b) Is encoded by the CD8B (also known as CD8B1) gene (Gene ID: 926) in human. CD8β is a single-pass type I membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-170), a transmembrane domain (aa 171-191), and a cytoplasmic domain (aa 192-210). Eight different isoforms of CD8β have been described that are generated by alternative splicing. Isoforms 1,3,5,6,7,8 are expressed in both thymus and peripheral CD8+ T cells. For efficient cell surface expression, it requires the CD8a as a partner and the heterodimer CD8a/CD8β localizes to lipid rafts due to CD8β cytoplasmic tail palmitoylation. In T-cells, it functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. It interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-cells. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1H21, recombinant monoclonal |
| 描述 | recombinant, expressed in HEK 293 cells |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt 23.72 kDa observed mol wt ~34 kDa |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | recombinant expression Learn more aboutAntibody Enhanced Validation |
| technique(s) | ELISA: suitable immunocytochemistry: suitable |
| 同位素/亚型 | IgG |
| 表位序列 | Extracellular domain |
| Protein ID登记号 | NP_004922.1 |
| UniProt登记号 | P10966 |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
Sigma-Aldrich
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