半岛bd体育手机客户端 说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特异性
Clone 1C20 is a ZooMAb?rabbit recombinant monoclonal antibody that specifically detects CaM Kinase IIa. It targets an epitope within 19 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human CaM Kinase IIa.
应用
Anti-CaM Kinase IIa, clone 1C20 ZooMAb?, Cat. No. ZRB1206, is a recombinant Rabbit monoclonal antibody that specifically targets CaM Kinase IIa and is tested in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in Rat Brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CaM Kinase IIa in Rat brain tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CaM Kinase IIa in Human Brain tissue lysate.
Affinity Binding Assay:: A representative lot of this antibody bound CaM Kinase IIa peptide with a KD of 2.2 x 10-7 in an affinity binding assay.
Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected CaM Kinase IIa in human cerebral cortex tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CaM Kinase IIa in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Calcium/calmodulin-dependent protein kinase type IIa (UniProt: Q9UQM7; also known as EC:2.7.11.17, CaM kinase II subunit alpha, CaMK-II subunit alpha) is encoded by the CAMK2A (also known as CAMKA, KIAA0968) gene (Gene ID: 815) in human. CaM kinase II is one of the most widely studied multi-functional enzyme. It is found in high concentrations in the brain and in some regions, it may constitute up to 2% of the total protein content. CaM kinase II is a complex of about 12 subunits, which exist in four differentially expressed forms (a, b, g, d). The a and b isoforms are most abundant in neurons, whereas d and g isoforms are expressed in most tissues. CaM Kinase II is activated by Ca2+/calmodulin. Binding of calmodulin to calmodulin binding domain (aa 290-300) results in conformational change that relieves intrasteric autoinhibition and allows autophosphorylation of Thr-286 which turns the kinase in a constitutively active form and confers to the kinase a Ca2+-independent activity. CaM Kinase II is shown to be involved in synaptic plasticity, neurotransmitter release, long-term potentiation, and migration of developing neurons. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外形
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 重组 | expressed in HEK 293 cells |
| 偶联物 | unconjugated |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 1C20, monoclonal recombinant monoclonal |
| 半岛bd体育手机客户端 线 | ZooMAb?learn more |
| 形式 | lyophilized |
| 分子量 | calculated mol wt ~ 54.09 kDa observed mol wt ~ 54 kDa |
| species reactivity | human, rat |
| species reactivity (predicted by homology) | mouse, bovine, monkey, canine |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| technique(s) | affinity binding assay: suitable immunofluorescence: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
