Anti-Bin1 Antibody, clone 99D.2 ZooMAb^?Mouse Monoclonal

半岛bd体育手机客户端 说明

一般描述

ZooMAb^?antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^?antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 99D.2 is a ZooMAb^?mouse recombinant monoclonal antibody that specifically detects Myc box-dependent-interacting protein 1 (Bin1). It targets an epitope within the internal region.

免疫原

Recombinant fragment corresponding to 210 amino acids from the Internal region of human Myc box-dependent-interacting protein 1 (Bin1).

应用

Quality Control Testing
Evaluated by Western Blotting in C2C12 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Bin1 in C2C12 cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Bin1 in human skeletal muscle tissue lysate.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Bin1 in C2C12 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Bin1 in human cerebellum and human skeletal muscle tissue sections.
Flow Cytometry Analysis: 1 μg from a representative lot detected Bin1 in one million HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Bin1, clone 99D.2 ZooMAb^?, Cat. No. ZMS1067, is a recombinant Mouse monoclonal antibody that detects Bin1 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.

目标描述

Myc box-dependent-interacting protein 1 (UniProt: O00499; also known as Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, Bin1) is encoded by the BIN1 (also known as AMPHL) gene (Gene ID: 274) in human. Bin1 is a ubiquitous protein that displays highest expression levels in the brain and muscle. Eleven isoforms of Bin1 have been described that are generated by alternate splicing. These isoforms are translated into proteins differently in neurons and astrocytes. Cultured neurons mostly express isoforms 1,3,5, and 7 while cultured astrocytes express isoforms 2,5,9, and 10. Isoform IIA is reported to be expressed only in the brain where it is concentrated n axon initial segments and nodes of Ranvier. Bin1 contains one BAR domain (aa 29-276) and one SH3 domain (aa 520-592). Bin1 interacts with the functionally critical Myc box regions at the N- terminus of the MYC oncoprotein and inhibits MYC-induced malignant cell transformation. Hence, it is considered as a tumor suppressor. Mutations in BIN1 gene are known to cause centronuclear myopathy that is characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. This ZooMAb^?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Taga, M., et al. (2020). Mol. Neurodegeneration. 15; Article 44).

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
重组	expressed in HEK 293 cells
偶联物	unconjugated
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	99D.2, recombinant monoclonal
描述	recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线	ZooMAb?learn more
形式	lyophilized
分子量	calculated mol wt 64.70 kDa observed mol wt ~58 kDa
species reactivity	mouse, human
species reactivity (predicted by homology)	rat
包装	antibody small pack of 25 μL
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
technique(s)	flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable
同位素/亚型	IgG1
表位序列	Internal
UniProt登记号	O00499
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable