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位置: 首页> 品牌> Sigma-Aldrich> Anti-ACE2-cytoplasmic Antibody, clone 1C17 ZooMAb?Rabbit Monoclonal

Anti-ACE2-cytoplasmic Antibody, clone 1C17 ZooMAb?Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1632
规格纯度
recombinant, expressed in HEK 293 cells
价格
2138.93 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-ACE2-cytoplasmic Antibody, clone 1C17 ZooMAb ?Rabbit Monoclonal
Angiotensin-converting enzyme 2;EC:3.4.17.23;Angiotensin-converting enzyme homolog;ACEH;Angiotensin-converting enzyme-related carboxypeptidase;ACE-related carboxypeptidase;Metalloprotease MPROT15
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ZooMAb?antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb?antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb?antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1C17 is a Rabbit recombinant monoclonal antibody that specifically detects Angiotensin-converting enzyme 2 (ACE2). It targets an epitope within 21 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal, cytoplasmic domain of human Angiotensin-converting enzyme 2 (ACE2).

应用

Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ACE2-cytoplasmic in HepG2 cell lysate.
Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 and 1:100 dilution of this antibody detected ACE2-cytoplasmic in human kidney and human lung tissue sections, respectively.
.
Affinity Binding Assay: A representative lot of this antibody bound ACE2-cytoplasmic domain with a KD of 10.0 x 10-9 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ACE2-cytoplasmic in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Angiotensin-converting enzyme 2 (UniProt: Q9BYF1; also known as EC:3.4.17.23, Angiotensin-converting enzyme homolog, ACEH, Angiotensin-converting enzyme-related carboxypeptidase, ACE-related carboxypeptidase, Metalloprotease MPROT15) is encoded by the ACE2 (also known as UNQ868/PRO1885) gene (Gene ID: 59272) in human. Angiotensin-converting enzyme (ACE) and its homolog ACE2 belong to the ACE family of dipeptidyl carboxydipeptidases and are known to serve two opposing functions. ACE cleaves angiotensin I to generate angiotensin II that binds to angiotensin receptor leading to constriction of blood vessels and hypertension. ACE2 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 18-740), a transmembrane domain (aa 741-761), and a cytoplasmic domain (aa 762-805). By contract, ACE2 is reported to inactivate angiotensin II while generating angiotensin 1 7, a heptapeptide with vasodilator function via activation of its Mas receptor. ACE2 expression in airway epithelial cells is induced by viruses such rhinoviruses, influenza virus, and SARS-CoV-2. SARS-CoV and SARS-CoV-2 utilize ACE2 as receptor for entry into cells and the receptor binding domain of SARS-Cov-2 displays strong interaction with human ACE2 molecules. The receptor binding domain (RBD) of spike protein serves as the binding interface with the ACE2 receptor. High ACE2 expression is reported in type II alveolar cells of lung, esophagus, absorptive enterocytes of ileum and colon, and kidney proximal tubule cells. These organs are considered as higher potential risk for SARS-CoV-2 infection. In animal models it is shown that treatment with Angiotensin converting enzyme inhibitors (ACEI) and Angiotensin receptor blockers (ARBs) can increase the expression of ACE2 in various organs and may increase their risk and severity of SARS-CoV-2 infection. This ZooMAb?recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yang, J., et al. (2020). Nat. Commun. 11(1); Article 4541; Hoffmann, M., et al. (2020). Cell 181(2); 271-280).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 rabbit
重组 expressed in HEK 293 cells
偶联物 unconjugated
抗体形式 purified antibody
antibody product type primary antibodies
克隆 1C17, recombinant monoclonal
描述 recombinant, expressed in HEK 293 cells
半岛bd体育手机客户端 线 ZooMAb?learn more
形式 lyophilized
分子量 calculated mol wt 92.46 kDa
observed mol wt ~130 kDa
纯化方式 using Protein A
species reactivity human
species reactivity (predicted by homology) monkey
包装 antibody small pack of 25 μL
环保替代半岛bd体育手机客户端 特性 Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about thePrinciples of Green Chemistry.
增强验证 recombinant expression
Learn more aboutAntibody Enhanced Validation
technique(s) affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型 IgG
表位序列 C-terminus
Protein ID登记号 NP_001358344
UniProt登记号 Q9BYF1
环保替代半岛bd体育手机客户端 分类 Aligned
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 13 - Non Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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