Anti-MDM4 Antibody, clone 7A8

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一般描述

MDM4 (sometimes referred to as MDMX) is a member of the MDM family proteins. It is known to inhibit p53 and p73 mediated cell cycle arrest and apoptosis. MDM4 is amplified or overexpressed in 10-20% of tumors and 65% of retinoblastomas. The full-length protein has significant homology to MDM2 and can also bind to and inhibit p53. In both MDM2 and MDM4, this interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Other similar domains include the C-terminal RING finger motif. Although these proteins share similar domains, MDM2 and MDM4 are non-redundant p53 inhibitors, since normal levels of either protein does not compensate for the loss of the other. Recent evidence suggests complementary activities for these proteins with MDM4 regulating p53 activity, and MDM2 playing a key role in p53 stability.

特异性

This antibody recognizes MDM4.

免疫原

His-tagged recombinant protein corresponding to mouse MDM4.
Epitope: Unknown

应用

Use Anti-MDM4 Antibody, clone 7A8 (mouse monoclonal antibody) validated in WB to detect MDM4 also known as Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse).
Research Sub Category
Transcription Factors

Apoptosis - Additional
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 ?g/ml of this antibody detected MDM4 in 10 ?g of HeLa cell lysate.

目标描述

~ 80/27 kDa Observed. There are multiple forms for MDM4. Up to 7 different variants have been reported. Full legth of MDM4 is ~80kDa The short form of MDM4 (MDM4-S) migrates at approximately 17 and 27kDa (R. Rallapalli, G. Strachan, B. Cho, W. Mercer?, and DJ. Hall, 1999, A Novel MDMX Transcript, THE JOURNAL OF BIOLOGICAL CHEMISTRY, 274(12), 8299–8308)

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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