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位置: 首页> 品牌> Sigma-Aldrich> Anti-HA Tag Antibody, clone DW2, rabbit monoclonal

Anti-HA Tag Antibody, clone DW2, rabbit monoclonal

品牌
Sigma-Aldrich
货号
04-902
规格纯度
culture supernatant, clone DW2, from rabbit
价格
7197.66 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-HA Tag Antibody, clone DW2, rabbit monoclonal
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

特异性

Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.
Reactivity with other species has not been confirmed.

免疫原

KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.

应用

Research Category
Epitope Tags & General Use
Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Research Sub Category
Epitope Tags
Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.

质量

Western Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.

目标描述

Various

联系

Replaces: 05-902

外形

Cultured supernatant in 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HA-tagged WNT-1 protein.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.43

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 culture supernatant
antibody product type primary antibodies
克隆 DW2, monoclonal
species reactivity vertebrates (common species)
technique(s) western blot: suitable
同位素/亚型 IgG
运输 dry ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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