Anti-Ago2 Antibody, clone 9E8.2

半岛bd体育手机客户端 说明

一般描述

Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).

特异性

human Ago2

免疫原

Epitope: near the N-terminus of Ago2
KLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG

应用

Anti-Ago2 Antibody, clone 9E8.2 is a Mouse Monoclonal Antibody for detection of Ago2 also known as Argonaute-2, Eukaryotic translation initiation factor 2C & has been validated in WB and has been demonstrated to perform in ChIP and ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Chromatin Biology

RNA Binding Protein (RBP)
ChIP and ChIP-seq: A representative lot of this antibody was used to perform ChIP and ChIP-seq ( Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294).

质量

Routinely evaluated by immunoblot.

目标描述

~100 kda

外形

ImmunoAffinity Purified
Ascites with 0.05% sodium azide

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	9E8.2, monoclonal
species reactivity	human
manufacturer/tradename	Upstate?
technique(s)	ChIP: suitable (ChIP-seq) western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NM_012154
UniProt登记号	Q9UKV8
运输	wet ice
Gene Information	human ... AGO2(27161)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable