Anti-SMN Antibody, clone 62E7

半岛bd体育手机客户端 说明

一般描述

The survival of motor neurons (SMN) protein is essential for the biogenesis of small nuclear RNA (snRNA)-ribonucleoproteins (snRNPs), the major components of the pre-mRNA splicing machinery. Though it is ubiquitously expressed, SMN deficiency causes the motor neuron degenerative disease spinal muscular atrophy (SMA). SMN deficiency, similar to that which occurs in severe SMA, has unexpected cell type-specific effects on the repertoire of snRNAs and mRNAs. It alters the stoichiometry of snRNAs and causes widespread pre-mRNA splicing defects in numerous transcripts of diverse genes, preferentially those containing a large number of introns, in SMN-deficient mouse tissues. These findings reveal a key role for the SMN complex in RNA metabolism and in splicing regulation and indicate that SMA is a general splicing disease that is not restricted to motor neurons.

特异性

Recognizes SMN.

免疫原

His-tagged recombinant protein corresponding to human SMN.
Epitope: Unknown

应用

Research Category
Epigenetics & Nuclear Function
Use Anti-SMN Antibody, clone 62E7 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect SMN also known as Spinal muscular atrophy (Werdnig-Hoffmann disease Kugelberg-Welander disease).
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SMN in HeLa cells.
Research Sub Category
RNA Metabolism & Binding Proteins

质量

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 ?g/ml of this antibody detected SMN on 10 ?g of HeLa cell lysate.

目标描述

35 kDa was observed; however, the calculated molecular weight is 31.849 kDa

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	62E7, monoclonal
species reactivity	human
species reactivity (predicted by homology)	Xenopus, mouse
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_000335.1
UniProt登记号	Q16637
运输	wet ice
Gene Information	human ... SMA4(11039)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable