Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal

半岛bd体育手机客户端 说明

一般描述

S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that is known to be phosphorylated by both p70S6 Kinase as well as RSK as well as Ser240/244 which is thought to only be phosphorylated by p70S6K.

特异性

Broad species cross-reactivity predicted, due to sequence homology.
Recognizes ribosomal protein S6 only when phosphorylated on Ser235.

免疫原

Synthetic peptide containing the sequence …RRL[pS]SL… in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus.
Epitope: Phosphorylated Ser235

应用

Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27 is an antibody against phospho-Ribosomal Protein S6 (Ser235) for use in WB.
Western Blot Analysis:
A 1:500-1:2,000 dilution of this lot detected phosphorylated ribosomal protein S6 in lysates from serum stimulated 3T3 cells.
Research Sub Category
PI3K, Akt, & mTOR Signaling
Research Category
Signaling

Apoptosis & Cancer

质量

Routinely evaluated by western blot on serum stimulated 3T3 cell lysate.

目标描述

~30 kDa

外形

Cultured supernatant with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20?C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Serum stimulated 3T3 cell lysate.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	culture supernatant
antibody product type	primary antibodies
克隆	MC27, monoclonal
species reactivity	human, rat, mouse
manufacturer/tradename	Upstate?
technique(s)	western blot: suitable
同位素/亚型	IgG
NCBI登记号	NM_001010.2
UniProt登记号	P62753
运输	dry ice
Gene Information	human ... RPS6(6194)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable