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位置: 首页> 品牌> Sigma-Aldrich> Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

品牌
Sigma-Aldrich
货号
04-791
规格纯度
culture supernatant, clone AW304, Upstate?
价格
6694.05 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal
H3K4me, Histone H3 (methyl K4)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone methylation is primarily associated with heterochromatin and gene silencing.

特异性

Monomethyl, dimethyl, and trimethyl histone H3 (Lys4). Additional unidentified bands above 50 kDa are detected in some samples
The immunizing sequence is conserved from Tetrahymena to human, so broad cross-reactivity is expected.

免疫原

Epitope: Lys4 of Histone H3
KLH-conjugated, synthetic peptide containing …Tme2KQT… in which me2K corresponds to dimethyl-lysine at residue 4 of human histone H3.

应用

Use Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304 (rabbit monoclonal antibody) validated in ChIP, WB, PIA, Mplex to detect mono/di/trimethyl-Histone H3 (Lys4) also known as H3K4me, Histone H3 (methyl K4).
Chromatin Immunoprecipitation:
The antibody was reported by an independent laboratory as suitable for ChIP.
Immunoblot Analysis:
A previous lot detected methylated Histone H3 in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant histone H3 (Catalog # 14-494).
Peptide Inhibition Analysis:
2 μM of a histone H3 peptide containing monomethyl-lysine 4, dimethyl lysine 4, and trimethyl-lysine 4 abolished detection of histone H3 by this lot in immunoblot analysis of HeLa acid extracts. Some signal reduction was observed with histone H3 peptides containing dimethyl-lysine 9 or 27.
Beadlyte Histone-Peptide Specificity Assay: 1:1,000-1:2,000 dilutions of this lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. Specificity for mono, di, and trimethyl-lysine 4 was demonstrated.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

质量

Routinely evaluated by immunoblot on nuclear extract from HeLa cells.
Immunoblot Analysis: A 1:500 (lane 1) and 1:1000 (lane 2) dilution of this lot detected methylated Histone H3 in HeLa nuclear extracts.

目标描述

~17 kDa

联系

Replaces: 05-791

外形

Cultured supernantant in 0.05% sodium azide

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa cell nuclear or acid extracts.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 culture supernatant
antibody product type primary antibodies
克隆 AW304, monoclonal
species reactivity human, vertebrates
manufacturer/tradename Upstate?
technique(s) ChIP: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NM_003493
UniProt登记号 Q16695
运输 dry ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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