半岛bd体育手机客户端 说明
一般描述
Insulin Receptor substrate proteins are the effectors of both Insulin and IGF-initiated signaling. They share PH and PTB domains near their n-termini, and multiple Tyr phosphorylation motifs in their c-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2.
特异性
IRS1
免疫原
Peptide corresponding to carboxy-terminal 14 amino acids (C-YASINFQKQPEDRQ) of rat liver IRS1
应用
Research Category
Signaling
Anti-IRS1 Antibody, clone AW58 detects level of IRS1 & has been published & validated for use in IP & WB.
Immunoblot Analysis: 1:1,000 to 1:4,000 dilutions of this lot detected IRS1 in RIPA lysates from 3T3/A31 mouse fibroblasts.
Immunoprecipitation: 5-10μl of this lot immunoprecipitated IRS1 from 500μg of 3T3/A31 RIPA lysate.
Research Sub Category
Insulin/Energy Signaling
质量
routinely evaluated by immunoblot on in RIPA lysates from 3T3/A31 mouse fibroblasts
目标描述
Mr ~145-165kDa
联系
Replaces: 05-784
外形
Cultured supernantant in 0.05% sodium azide
储存及稳定性
1 year at -20°C from date of shipment
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | culture supernatant |
| antibody product type | primary antibodies |
| 克隆 | AW58, monoclonal |
| species reactivity | human, mouse, rat |
| manufacturer/tradename | Upstate? |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG |
| NCBI登记号 | NM_005544 |
| UniProt登记号 | P35568 |
| 运输 | dry ice |
| Gene Information | human ... IRS1(3667) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
