Anti-Nestin Antibody, clone 10C2, Alexa Fluor^?488 conjugate

半岛bd体育手机客户端 说明

一般描述

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

特异性

This antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.

免疫原

Fusion protein corresponding to human Nestin.

应用

Research Category
Stem Cell Research

Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
Anti-Nestin Antibody, clone 10C2, Alexa Fluor 488 conjugate detects level of Nestin & has been published & validated for use in ICC.

质量

Evaluated by Immunocytochemistry in human RNcell CX neural stem cells (SCC007). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in human RNcell CX neural stem cells (SCC007).

目标描述

The uncojugated parent antibody (Catalog No. MAB5326) has an observed MW at 200-220 kDa

外形

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^?488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Protein A purified

储存及稳定性

Maintain refrigerated at 2-8?C protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析说明

Control
RNcell CX neural stem cells (SCC007).

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
偶联物	ALEXA FLUOR?488
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	10C2, monoclonal
species reactivity	human
technique(s)	immunocytochemistry: suitable
同位素/亚型	IgG1
NCBI登记号	NP_006608
UniProt登记号	P48681
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable