Monoclonal Anti-Ryanodine Receptor antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

The ryanodine receptor (RyR) is the channel responsible for the release of Ca²⁺from the sarcoplasmic reticulum (SR) in muscle cells and it also plays a role in Ca²⁺regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus.

免疫原

partially purified chicken pectoral muscle ryanodine receptors (RyR)

应用

Mouse monoclonal clone 34C anti-Ryanodine receptor is used to tag ryanodine receptors for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of ryanodine receptor in studies of calcium flux from the sarcoplasmic reticulum of muscle cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

外形

Purified IgG1 antibody containing 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00282461
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	34C, monoclonal
分子量	antigen 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms)
species reactivity	rabbit, fish, canine, sheep, frog, bovine, primate, mink, chicken, rat, human, mouse
technique(s)	direct immunofluorescence: suitable immunohistochemistry (frozen sections): 1:1,000 immunoprecipitation (IP): suitable western blot: 1:5,000
同位素/亚型	IgG1
UniProt登记号	P21817
运输	dry ice
储存温度	?20℃
Gene Information	human ... RYR1(6261), RYR2(6262) mouse ... Ryr1(20190), Ryr2(20191) rat ... Ryr1(114207), Ryr2(84025)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)