Anti-Histone H2A Antibody

半岛bd体育手机客户端 说明

一般描述

Histone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A’s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.

特异性

This antibody recognizes Histone H2A at the C-terminus.
Broad species reactivity expected based on sequence similarity.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.

应用

Use Anti-Histone H2A Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Histone H2A also known as Histone H2A type 1, H2A.1, Histone H2A/p.
Immunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H2A in liver and kidney tissues.

质量

Evaluated by Western Blot in HeLa acid extract and recombinant histone proteins.
Western Blot Analysis: 0.1 ?g/mL of this antibody detected Histone H2A on 10 ?g of HeLa acid extract and recombinant histone proteins.

目标描述

~16 kDa observed

外形

Affinity purified

分析说明

Control
HeLa acid extract and recombinant histone proteins

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, rat, mouse
technique(s)	immunohistochemistry: suitable western blot: suitable
NCBI登记号	NP_003500
UniProt登记号	P0C0S8
运输	wet ice
Gene Information	human ... HIST1H2AG(8969)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable