Monoclonal Anti-H⁺/K⁺ATPase (β Subunit) antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

The H⁺/K⁺-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5′-triphosphatase (ATPase) family. H⁺/K⁺-ATPase consists of a large transmembrane, catalytic α subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (β) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H⁺/K⁺ATPase (β subunit) binds to H⁺/K⁺-ATPase β subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the β subunit, including a 60-80kDa glycosylated protein, a 52kDa β subunit precursor and the 34kDa core peptide.
The antibody reacts with various forms of the H⁺/K⁺ATPase β subunit. It binds an epitope within amino acids 1-13 or 15-28 located on the cytoplasmic side of the β subunit. The antibody has been shown to inhibit the enzymatic activity of the H⁺/K⁺-ATPase and to alter the affinity of the cytoplasmic K⁺binding site. It may be used to localize and detect H⁺/K⁺ATPase β subunit.

免疫原

core 34 kDa H⁺/K⁺ATPase β subunit from deglycosylated pig gastric microsomes.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-H⁺/K⁺ATPase (β subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.

外形

Solution in phosphate buffered saline containing 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02096517
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	2G11, monoclonal
形式	buffered aqueous solution
分子量	antigen 60-80 kDa (glycosylated form)
species reactivity	mouse, bovine, ferret, rabbit, canine, pig, rat
technique(s)	immunohistochemistry (frozen sections): suitable indirect immunofluorescence: 1:2,000 western blot (chemiluminescent): 1:4,000
同位素/亚型	IgG1
UniProt登记号	P18597
运输	dry ice
储存温度	?20℃
Gene Information	mouse ... Atp4b(11945) rat ... Atp4b(24217)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)