半岛bd体育手机客户端 说明
一般描述
Benzonase?Nuclease is a geneticallyengineered endonuclease fromSerratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42℃ and requires1-2 mM Mg2+for activation. The enzyme is also active in the presence of ionic and non-ionicdetergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by > 150 mM monovalentcations, > 100 mM phosphate, > 100 mMammonium sulfate, or > 100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/l and at a high concentration (HC) of 250 U/l. Both preparations are free of detectable protease and have specific activity> 1 × 106U/mg protein. The > 99% puritygrade is tested for endotoxins and contains< 0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:< 0.25 EU/1,000 units.Purity:> 99% by SDS-PAGE.
Effective viscosity reduction and removal of nucleic acids from protein solutions
包装
10 ku in Glass bottle
应用
用于去除蛋白样品中的核酸。
生化/生理作用
消化天然或热变性的DNA和RNA。
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that causes a ΔA??? of 1.0 in 30 minutes, which corresponds to complete digestion of 37 ?g DNA.
法律信息
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
酶学委员会(EC)编号 | 3.1.30.2 ( BRENDA | IUBMB ) |
MDL编号 | MFCD00131010 |
半岛bd体育手机客户端 性质
质量水平 | 300 |
测定 | >99% (SDS-PAGE) |
manufacturer/tradename | Novagen? |
储存条件 | OK to freeze |
杂质 | <0.25 EU/kU Total endotoxin |
运输 | wet ice |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich