rLysozyme Solution

半岛bd体育手机客户端 说明

一般描述

rLysozyme Solution contains a highlypurified and stabilized recombinant lysozymethat can be used for lysis of Gram-negativebacteria, such asE. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) forE. colilysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction withBugBuster , BugBuster HT, and PopCulture Reagents. In the absence of protein extractionreagents, direct lysis ofE. colican be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/?l in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON X-100, pH 7.5. rLysozyme Solution is stable at –20°C.

警告

Toxicity: Standard Handling (A)

单位定义

One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A??? unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner (DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	400
manufacturer/tradename	Novagen?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
储存温度	?20℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2