半岛bd体育手机客户端 说明
一般描述
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction.
The RIPAb+ HuR set includes the HuR antibody, a negative control antibody (purified Rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human beta actin (ACTB) cDNA. The HuR and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation HuR-associated RNAs.
免疫原
Epitope: a.a. 1-13 of HuR
应用
Immunoprecipitation from RIP lysate:
RIP lysate from MCF7 cells (2 X 106cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-HuR antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HuR (1.0 μg/mL).
Proteins were visualized using One-Step
Western Blot Analysis:
3T3/A31 lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with HuR, hu (1:500 dilution). Proteins were visualized using a Donkey anti-Rabbit conjugated to HRP and visualized using a chemiluminescence detection system. (Please see figures).
Immunoprecipitation Analysis:
10 ug of this antibody immunoprecipitated HuR from 500 ug of 3T3/A31 RIPA lysate (Please see figures).
mmunocytochemistry Analysis:
Confocal IF analysis of HeLa, NIH/3T3 using anti-HuR (Red). Actin filaments have been labeled with AlexaFluor
This RIPAb+ HuR -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
质量
RNA binding protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 x 107cell equivalents per IP) were subjected to immunoprecipitation using 5 ?g of either a normal Rabbit IgG or Anti-HuR antibody and the Magna RIP Kit (Cat. # 17-700).
Successful immunoprecipitation of HuR-associated RNA was verified by qPCR using RIP Primers, ACTB (Please see figures).
Please refer to the Magna RIP?(Cat. # 17-700) or EZ-Magna RIP
目标描述
35 kDa
外形
Protein A purified
Anti-HuR (rabbit polyclonal). One vial containing 50 μg of purified rabbit polyclonal in 100 μL of buffer containing PBS in 0.05% sodium azide with 50% Glycerol. Liquid at -20°C.
Normal Rabbit IgG. One vial containing 125 μg purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
RIP Primers, ACTB. One vial containing 75 μL of 5 μM of each primer specific for human beta actin (ACTB). Store at -20°C.
FOR: TTG TTA CAG GAA GTC CCT TGC C
REV: ATG CTA TCA CCT CCC CTG TGT G
Format: Purified
分析说明
Control
Included negative control rabbit IgG antibody and control primers specific for human beta actin (ATCB).
法律信息
MAGNA RIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.32 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
克隆 | polyclonal |
species reactivity | mouse, rat, human |
manufacturer/tradename | RIPAb+ Upstate? |
technique(s) | RIP: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NP_001410 |
UniProt登记号 | Q15717 |
运输 | dry ice |
Gene Information | human ... ELAVL1(1994) |
安全信息
储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich