半岛bd体育手机客户端 说明
一般描述
NovaTaq DNA Polymerase* is a premium quality, recombinant form ofThermus aquaticusDNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. NovaTaqDNA Polymerase has an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt, AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaqBuffer with 15 mM MgCl2for routine amplification conditions, plus separate vials of 10X NovaTaqBuffer without MgCl2, and 25 mM MgCl2to enable convenient optimization of Mg2+concentration.
Source:Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration:5.0 U/μl
Purity:95% homogeneous by SDS-PAGE
Endonuclease:None detected
Amplification effiency:Functional PCR
Storage:–20℃
NovaTaqDNA polymerase is a recombinant form ofThermus aquaticusDNA polymerase. This enzyme is a non-proofreading DNA polymerase. NovaTaqDNA polymerase exhibits 5′-3′ DNA polymerase activity and lacks 3′- 5′ exonuclease activity. The preparation is >95% homogenous by sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) and lacks detectable endonuclease activities. It is tested for several quality control assays. This enzyme produces PCR products with 3′-dA overhangs, suitable for cloning with Novagen?Perfectly Blunt?, AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaqBuffer with 15 mM MgCl2for routine amplification conditions, plus separate vials of 10X NovaTaqBuffer without MgCl2, and 25 mM MgCl2to enable convenient optimization of Mg2+concentration.
Source:RecombinantThermus aquaticusDNA polymerase expressed inE. coli
Concentration:5.0 U/μl
Purity:95% homogeneous by SDS-PAGE
Endonuclease:None detected
Amplification effiency:Functional PCR
Storage:–20℃
应用
NovaTaqDNA polymerase has been used to check the success of the ligation step during cloning. It has also been used in the PCR amplification of cDNA.
NovaTaqDNA Polymerase can be used in standard polymerase chain reaction (PCR) amplification protocols. It is also suitable for colony PCR for screening bacterial colonies.
特点和优势
组分
?100 U, 500 U,or5 × 500 UNovaTaqDNA Polymerase
?1or2or7 × 1.5 ml10X NovaTaqBuffer with MgCl?
?1or2or7 × 1.5 ml10X NovaTaqBuffer without MgCl?
?1or2or7 × 1.5 ml25 mM MgCl?
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
单位定义
One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 74°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl?, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 ?M [α-32P]dCTP, and activated salmon sperm DNA.
法律信息
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 用途 | sufficient for 2000 reactions sufficient for 400 reactions sufficient for 80 reactions |
| 特点 | dNTPs included: no hotstart: no |
| manufacturer/tradename | Novagen? |
| 储存条件 | OK to freeze |
| technique(s) | PCR: suitable |
| input | purified DNA |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
Sigma-Aldrich
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