ColiComplete

半岛bd体育手机客户端 说明

一般描述

ColiComplete is a patented substrate supporting disc method containing both XGAL and MUG. It is designed to simultaneously detect Total Coliforms andE. coliin food products. The β-galactosidase enzyme produced by coliforms reacts with the chromogenic XGAL substrate to produce a visually detectable insoluble blue dye. The β-glucuronidase enzyme found inE. colireacts with the MUG substrate to produce a blue fluorescent reaction under long wave UV light.

应用

AOAC Official Method 992.30

特点和优势

• ColiComplete provides confirmed quantitativeE. coliresults in as few as 28 hours and confirmed total Coliforms result in 48 hours.
• Just add the patented ColiComplete disc to Lauryl Sulfate Broth (LST) tubes at the time of inoculation, incubate and read results. Use the Most Probably Number (MPN) format for quantitative results. No transfers are necessary.
• Simultaneous detection of bothE. coliand total Coliforms from a single test. Results are confirmed and require no subculturing.

组分

Bottle of reagent discs

原理

ColiComplete contains 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal) and 4-methyl umbelliferyl-β-D-glucuronide (MUG). Discs are added to LST inoculated with selected dilutions of samples. Samples are incubated at 35 - 37^oC and examined after 24 and 48 ± 2 h for confirmed total coliforms and after 30 ± 2 h for confirmedE. coliresults. β-Galactosidase, from coliforms present in samples, cleaves X-Gal into 5-bromo-4-chloro-indoxyl intermediate which undergoes oxidation to yield water-insoluble blue dimer, visually detectable on disc or in surrounding medium as confirmed positive result for total coliform activity. β-Glucuronidase, fromE. colipresent in samples, cleaves MUG into glucuronide and methyl umbelliferone, which fluoresces under long wave UV light (366 nm) as confirmed positive result forE. colipresence.
NOTE: AsE. coliO157:H7 does not produce β-glucuronidase, ColiComplete is not suitable for the detection ofE. coliO157:H7.

测试方法

X-Gal, MUG

法律信息

AOAC is a registered trademark of AOAC International

半岛bd体育手机客户端 性质

物料	paper disks (containing 5-bromo-4-chloro-3-indolyl-?-D-galactopyranoside (X-Gal) and 4-methyl umbelliferyl-?-D-glucuronide (MUG))
agency	AOAC?992.30 (OMA)
保质期	6?18 mo.
包装	pkg of 100 pkg of 3000
manufacturer/tradename	BioControl Systems 10800-100 BioControl Systems 10800-3K
application(s)	food and beverages pathogen testing
储存温度	2-8℃
适用性	coliforms:Escherichia coli

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable