KOD Hot Start Master Mix

半岛bd体育手机客户端 说明

一般描述

KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultra-pure deoxynucleotides, and reaction buffer with MgSO₄. The monoclonal antibodies inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures.
KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. It contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure dNTPs, and reaction buffer with MgSO4. The mix is ideal for use in high-throughput PCR applications.

应用

KOD Hot Start Master Mix has been used:

• To yield PCR product for T7 endonuclease I (T7EI) assay.
• For PCR amplification of the DNA isolated fromChrysosporium multifidum, a fungus with antimicrobial activity.
• In the PCR amplification of cDNA.
• In two-step PCR for the amplification of the target sequence followed by its insertion into a vector.

包装

500 reactions in Glass bottle
100 reactions in Plastic ampoule

特点和优势

• Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
• Amplifies genomic DNA templates up to 12 kbp
• Amplifies plasmid and lambda DNA template up to 21 kbp
• Eliminates mispriming and primer-dimer formation
• Convenient ambient-temperature setup compatible with automation
• KOD HotStart^?Buffer ensures optimal PCR performance over a wide range of targets.
• Offers high specificity, fidelity and yield during PCR amplification
• Optimized for ease of use and data reproducibility
• Simplifies PCR set-up and saves time
• High consistency with minimal risk of contamination

*Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).
Note:Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

组分

• 2 × 1.25 mlor10 × 1.25 mL KOD Hot Start Master Mix

References:
Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl?, 7.5 mM DTT, 50 ?g/ml BSA, 150 ?M each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), and 150 ?g/ml activated calf thymus DNA.

其他说明

Simply add KOD HotStart^?Master Mix to an equal volume of sample containing DNA template and primers. The final diluted reaction contains 1U KOD HotStart^?DNA Polymerase per 50 μl reaction. The smaller available size provides sufficient master mix for 100 (50 μl scale), or 250 (20 μl scale) reactions, while the larger size is adequate for 500 (50 μl scale) or 1250 (20 μl scale) reactions.

法律信息

* Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
形式	ready-to-use solution
用途	sufficient for 100 reactions sufficient for 500 reactions
特点	Difficult Templates/Specialty Enzymes PCR High Fidelity PCR dNTPs included hotstart
manufacturer/tradename	Novagen?
储存条件	OK to freeze
technique(s)	PCR: suitable (2X PCR Master Mix for high fidelity) PCR: suitable
input	purified DNA
运输	dry ice wet ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable