半岛bd体育手机客户端 说明
一般描述
Rosetta-gami 2host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed inE. coli. ThesetrxB/gormutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs.
Genotype:Δ(ara-leu)7697ΔlacX74ΔphoA PvuIIphoR araD139 ahpC galE galK rpsLF′[lac+lacIqpro]gor522::Tn10 trxBpRARE2 (CamR, StrR, TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
组分
0.4 ml1 mlComponent
?2 × 0.2 ml5 × 0.2 mlRosetta-gami 2 Competent Cells
?2 × 2 ml4 × 2 mlSOC Medium
?10 ?l10 ?lTest Plasmid
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
质量水平 | 100 |
manufacturer/tradename | Novagen? |
储存条件 | OK to freeze |
细胞转化 | transformation efficiency: 2×106cfu/μg |
储存温度 | ?70℃ |
Sigma-Aldrich